Primary endothelial cells isolated from the yolk sac and para-aortic splanchnopleura support the expansion of adult marrow stem cells in vitro

Weiming Li, Scott A. Johnson, William C. Shelley, Michael Ferkowicz, Paul Morrison, Yanjun Li, Mervin C. Yoder

Research output: Contribution to journalArticle

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Abstract

The embryonic origin and development of hematopoietic and endothelial cells is highly interdependent. We hypothesized that primary endothelial cells from murine yolk sac and para-aortic splanchnopleura (P-Sp) may possess the capacity to expand hematopoietic stem cells (HSCs) and progenitor cells ex vivo. Using Tie2-GFP transgenic mice in combination with fluorochrome-conjugated monoclonal antibodies to vascular endothelial growth factor receptor-2 (Flk1) and CD41, we have successfully isolated pure populations of primary endothelial cells from 9.5-days after coitus (dpc) yolk sac and P-Sp. Adult murine bone marrow Sca-1+c-Kit+lin- cells were cocultured with yolk sac or P-Sp Tie2-GFP+Flk-1+CD41- endothelial cell monolayers for 7 days and the total number of nonadherent cells increased 47- and 295-fold, respectively, and hematopoietic progenitor counts increased 9.4- and 11.4-fold, respectively. Both the yolk sac and P-Sp endothelial cell cocultures facilitated long-term (> 6 months) HSC competitive repopulating ability (2.8- to 9.8-fold increases, respectively). These data suggest that 9.5-dpc yolk sac- and P-Sp-derived primary Tie2-GFP +Flk-1+CD41- endothelial cells possess the capacity to expand adult bone marrow hematopoietic progenitor cell and HSC repopulating ability ex vivo.

Original languageEnglish (US)
Pages (from-to)4345-4353
Number of pages9
JournalBlood
Volume102
Issue number13
DOIs
StatePublished - Dec 15 2003

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Yolk Sac
Adult Stem Cells
Endothelial cells
Stem cells
Endothelial Cells
Bone Marrow
Hematopoietic Stem Cells
Aptitude
Bone
Vascular Endothelial Growth Factor Receptor-2
Coitus
Coculture Techniques
Fluorescent Dyes
Transgenic Mice
Embryonic Development
In Vitro Techniques
Monolayers
Stem Cells
Cell Count
Monoclonal Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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Primary endothelial cells isolated from the yolk sac and para-aortic splanchnopleura support the expansion of adult marrow stem cells in vitro. / Li, Weiming; Johnson, Scott A.; Shelley, William C.; Ferkowicz, Michael; Morrison, Paul; Li, Yanjun; Yoder, Mervin C.

In: Blood, Vol. 102, No. 13, 15.12.2003, p. 4345-4353.

Research output: Contribution to journalArticle

Li, Weiming ; Johnson, Scott A. ; Shelley, William C. ; Ferkowicz, Michael ; Morrison, Paul ; Li, Yanjun ; Yoder, Mervin C. / Primary endothelial cells isolated from the yolk sac and para-aortic splanchnopleura support the expansion of adult marrow stem cells in vitro. In: Blood. 2003 ; Vol. 102, No. 13. pp. 4345-4353.
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