Primary human leukocyte subsets differentially express vaccinia virus receptors enriched in lipid rafts

Daniel Byrd, Tohti Amet, Ningjie Hu, Jie Lan, Sishun Hu, Andy Yu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Poxviruses, including vaccinia virus (VV) and canarypox virus (ALVAC), do not indiscriminately infect all cell types of the primary human leukocytes (PHLs) that they encounter but instead demonstrate an extremely strong bias toward infection of monocytes and monocyte lineage cells. We studied the specific molecular events that determine the VV tropism for major PHL subsets including monocytes, B cells, neutrophils, NK cells, and T cells. We found that VV exhibited an extremely strong bias of cell surface protein-dependent binding to monocytes, B cells, and activated T cells to a similar degree and to neutrophils to a much lesser extent. Resting T cells and resting NK cells exhibited only trace amounts of VV binding. Activated T cells, however, became permissive to VV binding, infection, and replication, while activated NK cells still resisted VV binding. VV binding strongly colocalized with lipid rafts on the surfaces of all VV binding-susceptible PHL subsets, even when lipid rafts were relocated to cell uropods upon cell polarization. Immunosera raised against detergent-resistant membranes (DRMs) from mono-cytes or activated T cells, but not resting T cells, effectively cross-blocked VV binding to and infection of PHL subsets. CD29 and CD98, two lipid raft-associated membrane proteins that had been found to be important for VV entry into HeLa cells, had no effect on VV binding to and infection of primary activated T cells. Our data indicate that PHL subsets express VV protein receptors enriched in lipid rafts and that receptors are cross-presented on all susceptible PHLs.

Original languageEnglish
Pages (from-to)9301-9312
Number of pages12
JournalJournal of Virology
Volume87
Issue number16
DOIs
StatePublished - Aug 2013

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Virus Receptors
Vaccinia virus
leukocytes
Leukocytes
Virus Attachment
Lipids
lipids
T-lymphocytes
T-Lymphocytes
monocytes
Monocytes
natural killer cells
Virus Diseases
Natural Killer Cells
cells
infection
B-lymphocytes
Canarypox virus
virus receptors
neutrophils

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Primary human leukocyte subsets differentially express vaccinia virus receptors enriched in lipid rafts. / Byrd, Daniel; Amet, Tohti; Hu, Ningjie; Lan, Jie; Hu, Sishun; Yu, Andy.

In: Journal of Virology, Vol. 87, No. 16, 08.2013, p. 9301-9312.

Research output: Contribution to journalArticle

Byrd, Daniel ; Amet, Tohti ; Hu, Ningjie ; Lan, Jie ; Hu, Sishun ; Yu, Andy. / Primary human leukocyte subsets differentially express vaccinia virus receptors enriched in lipid rafts. In: Journal of Virology. 2013 ; Vol. 87, No. 16. pp. 9301-9312.
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