Primary human macrophages serve as vehicles for vaccinia virus replication and dissemination

Daniel Byrd, Nicole Shepherd, Jie Lan, Ningjie Hu, Tohti Amet, Kai Yang, Mona Desai, Andy Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human monocytic and professional antigen-presenting cells have been reported only to exhibit abortive infections with vaccinia virus (VACV). We found that monocyte-derived macrophages (MDMs), including granulocyte macrophage colony-stimulating factor (GM-CSF)-polarized M1 and macrophage colony-stimulating factor (M-CSF)-polarized M2, but not human AB serumderived cells, were permissive to VACV replication. The titers of infectious virions in both cell-free supernatants and cellular lysates of infected M1 and M2 markedly increased in a time-dependent manner. The majority of virions produced in permissive MDMs were extracellular enveloped virions (EEV), a secreted form of VACV associated with long-range virus dissemination, and were mainly found in the culture supernatant. Infected MDMs formed VACV factories, actin tails, virion-associated branching structures, and cell linkages, indicating that MDMs are able to initiate de novo synthesis of viral DNA and promote virus release. VACV replication was sensitive to inhibitors against the Akt and Erk1/2 pathways that can be activated by VACV infection and M-CSF stimulation. Classical activation of MDMs by lipopolysaccharide (LPS) plus gamma interferon (IFN-γ) stimulation caused no effect on VACV replication, while alternative activation of MDMs by interleukin-10 (IL-10) or LPS-plus-IL-1-β treatment significantly decreased VACV production. The IL-10-mediated suppression of VACV replication was largely due to Stat3 activation, as a Stat3 inhibitor restored virus production to levels observed without IL-10 stimulation. In conclusion, our data demonstrate that primary human macrophages are permissive to VACV replication. After infection, these cells produce EEV for long-range dissemination and also form structures associated with virions which may contribute to cell-cell spread.

Original languageEnglish
Pages (from-to)6819-6831
Number of pages13
JournalJournal of Virology
Volume88
Issue number12
DOIs
StatePublished - 2014

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Vaccinia virus
Virus Replication
virus replication
macrophages
Macrophages
virion
Virion
monocytes
interleukin-10
macrophage colony-stimulating factor
Interleukin-10
Macrophage Colony-Stimulating Factor
cells
viruses
lipopolysaccharides
Lipopolysaccharides
infection
Viruses
Virus Release
granulocyte-macrophage colony-stimulating factor

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Primary human macrophages serve as vehicles for vaccinia virus replication and dissemination. / Byrd, Daniel; Shepherd, Nicole; Lan, Jie; Hu, Ningjie; Amet, Tohti; Yang, Kai; Desai, Mona; Yu, Andy.

In: Journal of Virology, Vol. 88, No. 12, 2014, p. 6819-6831.

Research output: Contribution to journalArticle

Byrd, D, Shepherd, N, Lan, J, Hu, N, Amet, T, Yang, K, Desai, M & Yu, A 2014, 'Primary human macrophages serve as vehicles for vaccinia virus replication and dissemination', Journal of Virology, vol. 88, no. 12, pp. 6819-6831. https://doi.org/10.1128/JVI.03726-13
Byrd, Daniel ; Shepherd, Nicole ; Lan, Jie ; Hu, Ningjie ; Amet, Tohti ; Yang, Kai ; Desai, Mona ; Yu, Andy. / Primary human macrophages serve as vehicles for vaccinia virus replication and dissemination. In: Journal of Virology. 2014 ; Vol. 88, No. 12. pp. 6819-6831.
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