Primary structure and tissue-specific expression of human β- hydroxyisobutyryl-coenzyme A hydrolase

John W. Hawes, Jerzy Jaskiewicz, Yoshiharu Shimomura, Boli Huang, Jamie Bunting, Edwin T. Harper, Robert Harris

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

β-Hydroxyisobutyryl-CoA (HIBYL-CoA) hydrolase is responsible for the specific hydrolysis of HIBYL-CoA, a saline catabolite, as well as the hydrolysis of β-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism. We have obtained the amino acid sequences of several tryptic peptides derived from purified rat liver HIBYL-CoA hydrolase, and the NH2-terminal peptize sequence was matched to the translated sequence of a human expressed sequence tag present in the data base of the IMAGE Consortium (Lawrence Livermore National Laboratory, Livermore, CA). The complete nucleotide sequence and the deduced amino acid sequence showed no similarity to the sequences of well known thioesterases but showed significant homology to the enoyl-CoA hydratase/isomerase enzyme family. The cDNA fragment corresponding to the mature (processed) protein was expressed in Escherichia coli. The purified recombinant enzyme displayed substrate specificity very similar to that of the rat enzyme and was specifically bound by polyclonal antibodies raised against purified rat liver HIBYL-CoA hydrolase. Northern and Western blot analyses with various human tissues indicated predominant expression in liver, heart, and kidney, with discrepancies occurring in the amounts of HIBYL-CoA hydrolase mRNA compared to stably expressed protein in several tissues.

Original languageEnglish
Pages (from-to)26430-26434
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number42
DOIs
StatePublished - 1996

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Hydrolases
Coenzyme A
Tissue
Liver
Rats
Dodecenoyl-CoA Isomerase
Amino Acid Sequence
Hydrolysis
Enoyl-CoA Hydratase
Enzymes
Amino Acids
Isomerases
Expressed Sequence Tags
Propionates
Substrate Specificity
Metabolism
Northern Blotting
Escherichia coli
Proteins
Nucleotides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Primary structure and tissue-specific expression of human β- hydroxyisobutyryl-coenzyme A hydrolase. / Hawes, John W.; Jaskiewicz, Jerzy; Shimomura, Yoshiharu; Huang, Boli; Bunting, Jamie; Harper, Edwin T.; Harris, Robert.

In: Journal of Biological Chemistry, Vol. 271, No. 42, 1996, p. 26430-26434.

Research output: Contribution to journalArticle

Hawes, John W. ; Jaskiewicz, Jerzy ; Shimomura, Yoshiharu ; Huang, Boli ; Bunting, Jamie ; Harper, Edwin T. ; Harris, Robert. / Primary structure and tissue-specific expression of human β- hydroxyisobutyryl-coenzyme A hydrolase. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 42. pp. 26430-26434.
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