Primary structure of rabbit skeletal muscle glycogen synthase deduced from cDNA clones

W. Zhang, M. F. Browner, R. J. Fletterick, Anna De Paoli-Roach, Peter Roach

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The complete amino acid sequence of rabbit skeletal muscle glycogen synthase was deduced from cDNA clones with a composite length of 3317 bp. An mRNA of 3.6 kb was identified by Northern blot analysis of rabbit skeletal muscle RNA. The mRNA coded for a protein of 734 residues with a molecular weight of 83,480. The deduced NH2-terminal and COOH-terminal sequences corresponded to those reported for the purified protein, indicating the absence of any proteolytic processing. At the nucleotide level, the 5' untranslated and coding regions were 79 and 90% identical for rabbit and human muscle glycogen synthases, whereas the 3' untranslated regions were significantly less similar. The enzymes had 97% amino acid sequence identity. Interestingly, the NH2 and COOH termini of rabbit and human muscle glycogen synthase, the regions of phosphorylation, showed the greatest sequence variation (15 of 19 mismatches and two insertion/deletion events), which may indicate different evolutionary constraints in the regulatory and catalytic regions of the molecule.

Original languageEnglish
Pages (from-to)2532-2536
Number of pages5
JournalFASEB Journal
Volume3
Issue number13
StatePublished - 1989

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glycogen (starch) synthase
Glycogen Synthase
Muscle
skeletal muscle
Skeletal Muscle
Complementary DNA
Clone Cells
rabbits
clones
Rabbits
Amino Acid Sequence
amino acid sequences
Amino Acids
Muscles
muscles
Messenger RNA
Phosphorylation
Nucleic Acid Regulatory Sequences
5' Untranslated Regions
3' Untranslated Regions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Primary structure of rabbit skeletal muscle glycogen synthase deduced from cDNA clones. / Zhang, W.; Browner, M. F.; Fletterick, R. J.; De Paoli-Roach, Anna; Roach, Peter.

In: FASEB Journal, Vol. 3, No. 13, 1989, p. 2532-2536.

Research output: Contribution to journalArticle

Zhang, W. ; Browner, M. F. ; Fletterick, R. J. ; De Paoli-Roach, Anna ; Roach, Peter. / Primary structure of rabbit skeletal muscle glycogen synthase deduced from cDNA clones. In: FASEB Journal. 1989 ; Vol. 3, No. 13. pp. 2532-2536.
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