Primary structures and effects on gastrointestinal motility of tachykinins from the rainbow trout

J. Jensen, Kenneth Olson, J. M. Conlon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume265
Issue number4 34-4
StatePublished - 1993
Externally publishedYes

Fingerprint

Tachykinins
Gastrointestinal Motility
Trout
Oncorhynchus mykiss
Neurokinin A
Substance P
Intestines
Neuropeptides
Peptides
Stomach
Neurokinin-1 Receptors
Brain
Gastrointestinal Tract
Muscles

Keywords

  • fish
  • gastrointestinal motility
  • neurokinin A
  • neuropeptide-γ
  • substance P

ASJC Scopus subject areas

  • Physiology

Cite this

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abstract = "Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.",
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T1 - Primary structures and effects on gastrointestinal motility of tachykinins from the rainbow trout

AU - Jensen, J.

AU - Olson, Kenneth

AU - Conlon, J. M.

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N2 - Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.

AB - Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.

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