Priming of experimental anxiety by repeated subthreshold GABA blockade in the rat amygdala

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Blockade of GABAA receptor function in the area of the anterior basolateral amygdala of rats elicits physiological (increases in heart rate and blood pressure) and behavioral changes similar to symptoms of human anxiety states. Repeated subthreshold blockade of GABAA receptors in this region appears to result in a long-term 'priming' of these anxiety-like responses. The present study was conducted to characterize the 'priming' of the heart rate and blood pressure responses and to test if these 'primed' animals would show increases in anxiety responses. Male Wistar rats with arterial catheters placed for physiological measurements were implanted with chronic microinjection cannulae in the anterior basolateral amygdaloid nucleus (BLA) under pentobarbital anesthesia. Repeated daily injections of a subthreshold dose of bicuculline methiodide (GABAA receptor antagonist; BMI) into the BLA elicited 'priming' of physiological responses after 3-5 injections and this response was maintained for at least 6 weeks. The primed animals also showed increased anxiogenic responses to GABAA blockade in the BLA. The 'priming' of anxiety responses were clearly elicited before kindling of seizures as measured by EEG. These results suggest that this 'priming' phenomenon may be similar to kindling and long-term potentiation. This could be one potential mechanism for developing pathological emotional response, such as chronic, high levels of anxiety.

Original languageEnglish
Pages (from-to)250-259
Number of pages10
JournalBrain Research
Volume699
Issue number2
DOIs
StatePublished - Nov 20 1995

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Amygdala
gamma-Aminobutyric Acid
Anxiety
GABA-A Receptors
Heart Rate
Blood Pressure
GABA-A Receptor Antagonists
Injections
Long-Term Potentiation
Microinjections
Pentobarbital
Wistar Rats
Electroencephalography
Seizures
Catheters
Anesthesia

Keywords

  • Bicuculline methiodide
  • Conflict
  • Kindling
  • Long-term potentiation
  • Social interaction

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Priming of experimental anxiety by repeated subthreshold GABA blockade in the rat amygdala. / Sanders, S. K.; Morzorati, Sandra; Shekhar, Anantha.

In: Brain Research, Vol. 699, No. 2, 20.11.1995, p. 250-259.

Research output: Contribution to journalArticle

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