Primitive hematopoietic progenitor cells are present in peripheral blood autografts

J. Tong, A. M. Gianni, S. Siena, Edward Srour, M. Bregni, R. Hoffman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this report we have used an in vitro assay for long-term culture-initiating cells (LTC-IC) to detect primitive hematopoietic progenitor cells (HPC) in the peripheral blood (PB) of cancer patients who received high-dose cyclophosphamide (HD-CTX) followed by a combination of recombinant hematopoietic growth factors (C-HGF) including either interleukin-3 (IL-3)+ granulocyte colony-stimulating factor (G-CSF), IL-3+ granulocyte-macrophage colony-stimulating factor (GM-CSF) or IL-3/GM-CSF fusion protein (PIXY-321). In addition, we have developed a quantitative assay for cells capable of generating additional colony-forming cells (pre-CFC) as a means of determining primitive HPC present in mobilized PB cells. CD34+ human leukocyte A (HLA)-DR- cells isolated from the mobilized PB were capable of initiating long-term hematopoiesis in vitro that persisted for 10 weeks, while CD34+ HLA-DR- cells obtained from the nonmobilized PB or BM were capable of sustaining long-term hematopoiesis in vitro for only 4 weeks and 8 weeks, respectively. As determined by a limiting dilution analysis of mobilized PB CD34+ HLA-DR- cells, the frequency of pre-CFC was 4.3% (range, 1.0-8.3%) Pre-CFC comprised 0.01% (range, 0.001-0.02%) of mobilized PB mononuclear cells, and 151 pre-CFC were calculated to be present in one milliliter of mobilized PB (range, 20-310/ml). These results suggest that PB mononuclear cells collected by leukaphoresis following mobilization with HD-CTX + C-HGFs contain not only differentiated HPCs but also more primitive HPC.

Original languageEnglish (US)
Pages (from-to)351-363
Number of pages13
JournalBlood Cells
Volume20
Issue number2-3
StatePublished - 1994
Externally publishedYes

Fingerprint

Autografts
Hematopoietic Stem Cells
Interleukin-3
Blood Cells
Leukocytes
Hematopoiesis
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocyte Colony-Stimulating Factor
Cyclophosphamide
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
In Vitro Techniques
Neoplasms
Proteins

Keywords

  • hematopoietic stem cells
  • long-term culture-initiating cells
  • peripheral blood grafts

ASJC Scopus subject areas

  • Hematology

Cite this

Tong, J., Gianni, A. M., Siena, S., Srour, E., Bregni, M., & Hoffman, R. (1994). Primitive hematopoietic progenitor cells are present in peripheral blood autografts. Blood Cells, 20(2-3), 351-363.

Primitive hematopoietic progenitor cells are present in peripheral blood autografts. / Tong, J.; Gianni, A. M.; Siena, S.; Srour, Edward; Bregni, M.; Hoffman, R.

In: Blood Cells, Vol. 20, No. 2-3, 1994, p. 351-363.

Research output: Contribution to journalArticle

Tong, J, Gianni, AM, Siena, S, Srour, E, Bregni, M & Hoffman, R 1994, 'Primitive hematopoietic progenitor cells are present in peripheral blood autografts', Blood Cells, vol. 20, no. 2-3, pp. 351-363.
Tong J, Gianni AM, Siena S, Srour E, Bregni M, Hoffman R. Primitive hematopoietic progenitor cells are present in peripheral blood autografts. Blood Cells. 1994;20(2-3):351-363.
Tong, J. ; Gianni, A. M. ; Siena, S. ; Srour, Edward ; Bregni, M. ; Hoffman, R. / Primitive hematopoietic progenitor cells are present in peripheral blood autografts. In: Blood Cells. 1994 ; Vol. 20, No. 2-3. pp. 351-363.
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