Prior cryopreservation of ex vivo-expanded cord blood cells is not detrimental to engraftment as measured in the NOD-SCID mouse model

A. M. Rice, J. A. Wood, C. G. Milross, C. J. Collins, Jamie Case, R. E. Nordon, M. R. Vowels

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cytokine-mediated expansion has been proposed and successfully used to facilitate engraftment post transplantation. This study examined whether cryopreservation following expansion has a detrimental effect on the ability of cells to engraft, using the NOD-SCID mouse model. Cord blood (CB) CD34+ cells were incubated for 7 days with stem cell factor (SCF), fit-3 ligand (FL), and megakaryocyte growth and development factor (MGDF). Expanded CD34+ cells were transplanted into NOD-SCID mice either fresh or following cryopreservation and thawing. After thawing, recovery of nucleated cells was 94%, of CD34 cells was 63%, and of day-14 progenitors was 17%. The loss of day-14 progenitor cells among the thawed expanded cells did not influence the kinetics of human engraftment in the mouse. Bone marrow (BM) of mice transplanted with thawed expanded CD34+ cells (14 ± 3.9%) showed significantly higher levels of human engraftment than mice transplanted with fresh expanded CD34+ cells (1.5 ± 0.5%, p = 0.0064). Thawed expanded CD34+ cells had significantly higher SCID Engrafting Potential (SEP) than freshly expanded CD34+ cells (p <0.001). Results suggest that prior cryopreservation does not prevent expanded cells engrafting in NOD-SCID mice.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Hematotherapy and Stem Cell Research
Volume10
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

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Inbred NOD Mouse
SCID Mice
Cryopreservation
Fetal Blood
Blood Cells
Thrombopoietin
Stem Cell Factor
Stem Cells
Transplantation
Bone Marrow
Cytokines
Ligands

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

Prior cryopreservation of ex vivo-expanded cord blood cells is not detrimental to engraftment as measured in the NOD-SCID mouse model. / Rice, A. M.; Wood, J. A.; Milross, C. G.; Collins, C. J.; Case, Jamie; Nordon, R. E.; Vowels, M. R.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 10, No. 1, 2001, p. 157-165.

Research output: Contribution to journalArticle

Rice, A. M. ; Wood, J. A. ; Milross, C. G. ; Collins, C. J. ; Case, Jamie ; Nordon, R. E. ; Vowels, M. R. / Prior cryopreservation of ex vivo-expanded cord blood cells is not detrimental to engraftment as measured in the NOD-SCID mouse model. In: Journal of Hematotherapy and Stem Cell Research. 2001 ; Vol. 10, No. 1. pp. 157-165.
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