Prior exercise enhances passive absorption of intraduodenal glucose

R. Richard Pencek, Yoshiharu Koyama, D. Brooks Lacy, Freyja D. James, Patrick T. Fueger, Kareem Jabbour, Phillip E. Williams, David H. Wasserman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The purpose of this study was to assess whether a prior bout of exercise enhances passive gut glucose absorption. Mongrel dogs had sampling catheters, infusion catheters, and a portal vein flow probe implanted 17 days before an experiment. Protocols consisted of either 150 min of exercise (n = 8) or rest (n = 7) followed by basal (-30 to 0 min) and a primed (150 mg/kg) intraduodenal glucose infusion [8.0 mg·kg-1·min-1, time (t) = 0-90 min] periods. 3-O-[3H]methylglucose (absorbed actively, facilitatively, and passively) and L-[14C]glucose (absorbed passively) were injected into the duodenum at t = 20 and 80 min. Phloridzin, an inhibitor of the active sodium glucose cotransporter-1 (SGLT-1), was infused (0.1 mg·kg-1·min-1) into the duodenum from t = 60-90 min with a peripheral venous isoglycemic clamp. Duodenal, arterial, and portal vein samples were taken every 10 min during the glucose infusion, as well as every minute after each tracer bolus injection. Net gut glucose output in exercised dogs increased compared with that in the sedentary group (5.34 ± 0.47 and 4.02 ± 0.53 mg·kg1·min -1). Passive gut glucose absorption increased ∼100% after exercise (0.93 ± 0.06 and 0.45 ± 0.07 mg·kg -·min-1). Transport-mediated glucose absorption increased by ∼20%, but the change was not significant. The infusion of phloridzin eliminated the appearance of both glucose tracers in sedentary and exercised dogs, suggesting that passive transport required SGLT-1-mediated glucose uptake. This study shows 1) that prior exercise enhances passive absorption of intraduodenal glucose into the portal vein and 2) that basal and the added passive gut glucose absorption after exercise is dependent on initial transport of glucose via SGLT-1.

Original languageEnglish (US)
Pages (from-to)1132-1138
Number of pages7
JournalJournal of Applied Physiology
Volume95
Issue number3
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Glucose
Sodium-Glucose Transporter 1
Portal Vein
Phlorhizin
Dogs
Duodenum
Catheters
3-O-Methylglucose
Injections
Gastrointestinal Absorption

Keywords

  • Dogs
  • Phloridzin
  • Splanchnic blood flow

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Pencek, R. R., Koyama, Y., Lacy, D. B., James, F. D., Fueger, P. T., Jabbour, K., ... Wasserman, D. H. (2003). Prior exercise enhances passive absorption of intraduodenal glucose. Journal of Applied Physiology, 95(3), 1132-1138.

Prior exercise enhances passive absorption of intraduodenal glucose. / Pencek, R. Richard; Koyama, Yoshiharu; Lacy, D. Brooks; James, Freyja D.; Fueger, Patrick T.; Jabbour, Kareem; Williams, Phillip E.; Wasserman, David H.

In: Journal of Applied Physiology, Vol. 95, No. 3, 01.09.2003, p. 1132-1138.

Research output: Contribution to journalArticle

Pencek, RR, Koyama, Y, Lacy, DB, James, FD, Fueger, PT, Jabbour, K, Williams, PE & Wasserman, DH 2003, 'Prior exercise enhances passive absorption of intraduodenal glucose', Journal of Applied Physiology, vol. 95, no. 3, pp. 1132-1138.
Pencek RR, Koyama Y, Lacy DB, James FD, Fueger PT, Jabbour K et al. Prior exercise enhances passive absorption of intraduodenal glucose. Journal of Applied Physiology. 2003 Sep 1;95(3):1132-1138.
Pencek, R. Richard ; Koyama, Yoshiharu ; Lacy, D. Brooks ; James, Freyja D. ; Fueger, Patrick T. ; Jabbour, Kareem ; Williams, Phillip E. ; Wasserman, David H. / Prior exercise enhances passive absorption of intraduodenal glucose. In: Journal of Applied Physiology. 2003 ; Vol. 95, No. 3. pp. 1132-1138.
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