Production of an antibody to mouse salivary androgen binding protein (ABP) and its use in identifying a prostate protein produced by a gene distinct from Abp

Stephen Dlouhy, William C. Nichols, Robert C. Karn

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We previously demonstrated polymorphism of a mouse salivary protein which, because of its ability to bind androgen, we designated androgen binding protein (ABP) and its structural gene, Androgen binding protein (Abp). This report describes the purification of salivary ABP and presents the amino acid composition of its subunits. Using an antibody raised against the purified protein, we demonstrated the presence of cross-reactive material (CRM) in mouse parotid, submaxillary, sublingual, and prostate glands by double immunodiffusion. Immunohistochemical detection of proteins on electroblots of polyacrylamide electrophoresis and isoelectric focusing gels, however shows that the prostate CRM is a protein with electrophoretic and isoelectric focusing behavior distinct from that of salivary ABP isoproteins. Because the DBA/2J strain has a variant of salivary ABP, that strain was analyzed to determine if a prostate ABP-CRM variant was present. The approach was hampered by an inability to detect CRM in the prostates of DBA/2J mice. Prostate CRM was detected, however, in some progeny from repeated backcrosses of DBA/2J × C3H/St hybrids to the C3H/St and DBA/2J parental strains. The prostate CRM detected in samples from animals heterozygous for salivary Abp appears to be identical to C3H/St prostate CRM, suggesting that the gene controlling prostate ABP-CRM is related to, but distinct from, Abp. The reason for reduced or absent CRM in the prostates of DBA/2J males is unknown but this finding suggests that there are strain-related differences in the expression of this prostate protein.

Original languageEnglish
Pages (from-to)743-763
Number of pages21
JournalBiochemical Genetics
Volume24
Issue number9-10
DOIs
StatePublished - Oct 1986

Fingerprint

Androgen-Binding Protein
androgen
antibody formation
androgens
antibody
Antibody Formation
binding proteins
Prostate
Genes
protein
Antibodies
gene
mice
Proteins
genes
proteins
isoelectric focusing
Isoelectric Focusing
Salivary Proteins and Peptides
Sublingual Gland

Keywords

  • androgen binding proteins
  • mouse prostate
  • mouse salivary proteins
  • Western transfer

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Production of an antibody to mouse salivary androgen binding protein (ABP) and its use in identifying a prostate protein produced by a gene distinct from Abp. / Dlouhy, Stephen; Nichols, William C.; Karn, Robert C.

In: Biochemical Genetics, Vol. 24, No. 9-10, 10.1986, p. 743-763.

Research output: Contribution to journalArticle

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