Production of [Asn1,Val5] angiotensin II and [Asp1 ,Val5] angiotensin II in kallikrein-treated trout plasma (T60K)

J. Michael Conlon, Kenji Yano, Kenneth Olson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Incubation of heat-denatured plasma from the rainbow trout Oncorhynchus mykiss with porcine pancreatic kallikrein generates, in addition to bradykinin-related peptides, previously uncharacterized peptides that contract mammalian and amphibian vascular smooth muscle. Using rings of vascular smooth muscle from the bullfrog systemic arch as bioassay, we have isolated two myotropic peptides whose primary structures were established as: Asn-Arg-Val-Tyr-Val-His-Pro-Phe ([Asn1,Val5]angiotensin II) and Asp-Arg-Val-Tyr-Val-His-Pro-Phe ([Asp1,Val5]angiotensin II). These peptides are the same as those generated in salmon plasma by an extract of kidney. The data raise the possibility that activation of the kallikrein-kinin system in trout generates both bradykinin-related and angiotensin II-related peptides that may act synergistically in the regulation of blood pressure.

Original languageEnglish (US)
Pages (from-to)527-530
Number of pages4
JournalPeptides
Volume17
Issue number3
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Kallikreins
Trout
Angiotensin II
Plasmas
Peptides
Oncorhynchus mykiss
Bradykinin
Vascular Smooth Muscle
Muscle
Kallikrein-Kinin System
Tissue Kallikreins
Rana catesbeiana
Kinins
Bioassay
Salmon
Blood pressure
Arches
Amphibians
Biological Assay
Swine

Keywords

  • Angiotensin II
  • Bradykinin
  • Kallikrein
  • Renin
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Production of [Asn1,Val5] angiotensin II and [Asp1 ,Val5] angiotensin II in kallikrein-treated trout plasma (T60K). / Conlon, J. Michael; Yano, Kenji; Olson, Kenneth.

In: Peptides, Vol. 17, No. 3, 1996, p. 527-530.

Research output: Contribution to journalArticle

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