Production of colonization factor antigen II of enterotoxigenic Escherichia coli is subject to catabolite repression

Dolores G. Evans, Doyle J. Evans, Tuomo K. Karjalainen, Chao Hung Lee

Research output: Contribution to journalArticle

7 Scopus citations


Experiments were performed to study the effect of glucose on the production of the fimbrial colonization factor CFA/II of enterotoxigenic Escherichia coli (ETEC). The production of the CFA/II antigen was examined by electron microscopy, quantitative ELISA, and hemagglutination. The results showed that addition of 1% glucose to the growth medium drastically decreased CFA/II production, whereas addition of glycerol or sodium acetate did not have any effect. Bacteria grown in the presence of 1% glucose were essentially devoid of CFA/II fimbriae when examined under the electron microscope. Addition of 1 m M cAMP reversed the repressive effect of glucose, suggesting that the glucose suppression on CFA/II synthesis is via the mechanism of catabolite repression.

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalCurrent Microbiology
Issue number2
StatePublished - Aug 1 1991


ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this