Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells

Christiane A. Opitz, Neta Rimmerman, Yanmin Zhang, Laura E. Mead, Mervin C. Yoder, David A. Ingram, J. Michael Walker, Jalees Rehman

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-α stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-α.

Original languageEnglish (US)
Pages (from-to)4927-4931
Number of pages5
JournalFEBS Letters
Volume581
Issue number25
DOIs
StatePublished - Oct 16 2007

Keywords

  • Endocannabinoid
  • Endothelial progenitor cell
  • Endothelium
  • Endothelium-derived factor
  • Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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