Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells

Christiane A. Opitz, Neta Rimmerman, Yanmin Zhang, Laura E. Mead, Mervin Yoder, David Ingram, J. Michael Walker, Jalees Rehman

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-α stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-α.

Original languageEnglish
Pages (from-to)4927-4931
Number of pages5
JournalFEBS Letters
Volume581
Issue number25
DOIs
StatePublished - Oct 16 2007

Fingerprint

Endocannabinoids
Endothelial cells
Vascular Cell Adhesion Molecule-1
Tandem Mass Spectrometry
Intercellular Signaling Peptides and Proteins
Endothelial Cells
High Pressure Liquid Chromatography
Mass spectrometry
Lipids
Blood
Adhesion
2-arachidonylglycerol
anandamide
Endothelial Progenitor Cells
Molecules

Keywords

  • Endocannabinoid
  • Endothelial progenitor cell
  • Endothelium
  • Endothelium-derived factor
  • Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells. / Opitz, Christiane A.; Rimmerman, Neta; Zhang, Yanmin; Mead, Laura E.; Yoder, Mervin; Ingram, David; Walker, J. Michael; Rehman, Jalees.

In: FEBS Letters, Vol. 581, No. 25, 16.10.2007, p. 4927-4931.

Research output: Contribution to journalArticle

Opitz, Christiane A. ; Rimmerman, Neta ; Zhang, Yanmin ; Mead, Laura E. ; Yoder, Mervin ; Ingram, David ; Walker, J. Michael ; Rehman, Jalees. / Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells. In: FEBS Letters. 2007 ; Vol. 581, No. 25. pp. 4927-4931.
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