Proficiency testing for laboratories performing fluorescence in situ hybridization with chromosome-specific DNA probes

James T. Mascarello, Arthur R. Brothman, Keri Davison, Gordon W. Dewald, Marille Herrman, Danette McCandless, Jonathan P. Park, Diane L. Persons, Kathleen W. Rao, Nancy R. Schneider, Gail H. Vance, Linda D. Cooley

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objective. - To assess laboratory performance, use, and limitations in the joint College of American Pathologists and American College of Medical Genetics proficiency testing program for laboratories performing cytogenetic tests based on fluorescence in situ hybridization (FISH). Data Sources. - Eight proficiency surveys dealing with FISH detection of microdeletions or microduplications, aneuploidy in interphase cells, gene amplification, and neoplasm-specific translocations. Participating laboratories used their own DNA probes (commercial or home-brew), hybridization methods, and analytic criteria to answer clinical questions about cases represented by slides included in the survey materials. They also described their test results according to the International System for Human Cytogenetic Nomenclature (ISCN) and answered supplementary questions relating to their experience with the subject test systems. Data Extraction. - In addition to evaluating diagnostic accuracy, we evaluated survey use, laboratory experience, variation in methodologic approach, and the practicality of using ISCN nomenclature for describing test results. Synthesis and Conclusions. - With the exception of one challenge, at least 80% of the participants reached the correct diagnostic conclusion. In the sole exception, there was still a consensus of 91.7% of participants with the same (albeit erroneous) diagnostic conclusion. The overall outstanding performance of participating laboratories clearly shows the reliability of current FISH methods. Despite the fact that a large number of laboratories reported little or no experience with the specific test systems, the overwhelming majority performed very well. This result shows that the program's strategy of targeting classes of abnormalities (vs a single abnormality associated with a specific disease) did not put at a disadvantage participants who did not routinely perform all of the potential tests in the class. The extraordinary variation in ISCN descriptions submitted by participants showed that the existing system for human cytogenetic nomenclature is not suitable for facile communication of FISH test results.

Original languageEnglish (US)
Pages (from-to)1458-1462
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume126
Issue number12
StatePublished - Dec 1 2002

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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