Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Xiongshan Sun, Qi Han, Hongqin Luo, Xiaodong Pan, Yan Ji, Yao Yang, Hanying Chen, Fangjie Wang, Wenjing Lai, Xiao Guan, Qi Zhang, Yuan Tang, Jianhong Chu, Jianhua Yu, Weinian Shou, Youcai Deng, Xiaohui Li

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mammalian cardiomyocytes undergo a critical hyperplastic-to-hypertrophic growth transition at early postnatal age, which is important in establishing normal physiological function of postnatal hearts. In the current study, we intended to explore the role of long non-coding (lnc) RNAs in this transitional stage. We analyzed lncRNA expression profiles in mouse hearts at postnatal day (P) 1, P7 and P28 via microarray. We identified 1,146 differentially expressed lncRNAs with more than 2.0-fold change when compared the expression profiles of P1 to P7, P1 to P28, and P7 to P28. The neighboring genes of these differentially expressed lncRNAs were mainly involved in DNA replication-associated biological processes. We were particularly interested in one novel cardiac-enriched lncRNA, ENSMUST00000117266, whose expression was dramatically down-regulated from P1 to P28 and was also sensitive to hypoxia, paraquat, and myocardial infarction. Knockdown ENSMUST00000117266 led to a significant increase of neonatal mouse cardiomyocytes in G0/G1 phase and reduction in G2/M phase, suggesting that ENSMUST00000117266 is involved in regulating cardiomyocyte proliferative activity and is likely associated with hyperplastic-to-hypertrophic growth transition. In conclusion, our data have identified a large group of lncRNAs presented in the early postnatal mouse heart. Some of these lncRNAs may have important functions in cardiac hyperplastic-to-hypertrophic growth transition.

Original languageEnglish (US)
Article number43485
JournalScientific Reports
Volume7
DOIs
StatePublished - Mar 7 2017

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Long Noncoding RNA
Cardiac Myocytes
Growth
Biological Phenomena
Cell Cycle Resting Phase
Paraquat
G2 Phase
G1 Phase
DNA Replication
Cell Division
Myocardial Infarction

ASJC Scopus subject areas

  • General

Cite this

Sun, X., Han, Q., Luo, H., Pan, X., Ji, Y., Yang, Y., ... Li, X. (2017). Profiling analysis of long non-coding RNAs in early postnatal mouse hearts. Scientific Reports, 7, [43485]. https://doi.org/10.1038/srep43485

Profiling analysis of long non-coding RNAs in early postnatal mouse hearts. / Sun, Xiongshan; Han, Qi; Luo, Hongqin; Pan, Xiaodong; Ji, Yan; Yang, Yao; Chen, Hanying; Wang, Fangjie; Lai, Wenjing; Guan, Xiao; Zhang, Qi; Tang, Yuan; Chu, Jianhong; Yu, Jianhua; Shou, Weinian; Deng, Youcai; Li, Xiaohui.

In: Scientific Reports, Vol. 7, 43485, 07.03.2017.

Research output: Contribution to journalArticle

Sun, X, Han, Q, Luo, H, Pan, X, Ji, Y, Yang, Y, Chen, H, Wang, F, Lai, W, Guan, X, Zhang, Q, Tang, Y, Chu, J, Yu, J, Shou, W, Deng, Y & Li, X 2017, 'Profiling analysis of long non-coding RNAs in early postnatal mouse hearts', Scientific Reports, vol. 7, 43485. https://doi.org/10.1038/srep43485
Sun, Xiongshan ; Han, Qi ; Luo, Hongqin ; Pan, Xiaodong ; Ji, Yan ; Yang, Yao ; Chen, Hanying ; Wang, Fangjie ; Lai, Wenjing ; Guan, Xiao ; Zhang, Qi ; Tang, Yuan ; Chu, Jianhong ; Yu, Jianhua ; Shou, Weinian ; Deng, Youcai ; Li, Xiaohui. / Profiling analysis of long non-coding RNAs in early postnatal mouse hearts. In: Scientific Reports. 2017 ; Vol. 7.
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