Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas

Fabio Puglisi, Fabio Barbone, Gianluca Tell, Giuseppe Aprile, Barbara Pertoldi, Concetta Raiti, Mark Kelley, Giuseppe Damante, Alberto Sobrero, Carlo Alberto Beltrami, Carla Di Loreto

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Abstract

The purpose of the study was to evaluate the prognostic role of the DNA repair protein APE/Ref-1 in breast carcinomas. Immunohistochemical analysis for APE/Ref-1 was performed in a series of 133 consecutive stage I-III breast carcinomas. The relationship between APE/Ref-1 and other prognostic and predictive factors such as tumor size, nodal status, histologic grade, p53 expression, hormonal receptor status, vascular invasion and necrosis was investigated. The prognostic value of APE/Ref-1 was studied by univariate and multivariate analysis. The predominant pattern of APE/Ref-1 immunohistochemical expression was nuclear, although cytoplasmic and mixed nuclear/cytoplasmic localization was also observed. The percentage of cells with APE/Ref-1 cytoplasmic stain directly correlated with the percentage of p53 positive cases (rho=0.28, p=0.013). The small group of women whose tumors showed mixed nuclear/cytoplasmic APE/Ref-1 localization (n=5) experienced a significantly poorer survival (p=0.014) and Cox proportional hazard model analysis identified APE/Ref-1 as an independent prognostic factor. The results suggest that subcellular localisation of APE/Ref-1 may influence the aggressiveness of breast carcinomas.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalOncology Reports
Volume9
Issue number1
StatePublished - Jan 2002

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Hominidae
Breast Neoplasms
Proportional Hazards Models
DNA Repair
Blood Vessels
Neoplasms
Necrosis
Coloring Agents
Multivariate Analysis
Survival
Proteins

Keywords

  • Breast carcinoma
  • DNA repair
  • Immunohistochemistry
  • Multivariate analysis
  • Prognosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Puglisi, F., Barbone, F., Tell, G., Aprile, G., Pertoldi, B., Raiti, C., ... Di Loreto, C. (2002). Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas. Oncology Reports, 9(1), 11-17.

Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas. / Puglisi, Fabio; Barbone, Fabio; Tell, Gianluca; Aprile, Giuseppe; Pertoldi, Barbara; Raiti, Concetta; Kelley, Mark; Damante, Giuseppe; Sobrero, Alberto; Beltrami, Carlo Alberto; Di Loreto, Carla.

In: Oncology Reports, Vol. 9, No. 1, 01.2002, p. 11-17.

Research output: Contribution to journalArticle

Puglisi, F, Barbone, F, Tell, G, Aprile, G, Pertoldi, B, Raiti, C, Kelley, M, Damante, G, Sobrero, A, Beltrami, CA & Di Loreto, C 2002, 'Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas', Oncology Reports, vol. 9, no. 1, pp. 11-17.
Puglisi F, Barbone F, Tell G, Aprile G, Pertoldi B, Raiti C et al. Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas. Oncology Reports. 2002 Jan;9(1):11-17.
Puglisi, Fabio ; Barbone, Fabio ; Tell, Gianluca ; Aprile, Giuseppe ; Pertoldi, Barbara ; Raiti, Concetta ; Kelley, Mark ; Damante, Giuseppe ; Sobrero, Alberto ; Beltrami, Carlo Alberto ; Di Loreto, Carla. / Prognostic role of Ape/Ref-1 subcellular expression in stage I-III breast carcinomas. In: Oncology Reports. 2002 ; Vol. 9, No. 1. pp. 11-17.
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