Prognostic significance of cellular proliferation in renal cell carcinoma: A comparison of synthesis-phase fraction and proliferating cell nuclear antigen index

David Grignon, M. Abdel-Malak, W. Mertens, J. Koster, M. Keeney, W. Sakr, R. R. Shepherd

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA aneuploidy has repeatedly been shown to be a significant prognostic indicator in renal cell carcinoma; however, few studies have emphasized the importance of measurements of cellular proliferation. This study evaluated 55 patients treated by radical nephrectomy and for whom clinical follow-up was available. There were 36 men and 19 women with a mean age of 61 yr. Robson stage distribution was I, 38 cases; II, five cases; and III, 12 cases. Flow cytometric analysis in 44 cases revealed 29 DNA diploid and 15 DNA aneuploid tumors with a median synthesis phase fraction (SPF) of 4.4% (range 1.0 to 31.4%). The median proliferating cell nuclear antigen index was 3.9% (range 0.1 to 58.0%). There was a significant correlation between SPF and proliferating cell nuclear antigen index (R = 0.769) in the 33 cases in which both were available. Cellular proliferation, as determined by SPF, was a significant prognostic indicator (P <0.02), but proliferating cell nuclear antigen index did not correlate with outcome (P > 0.05). Other significant predictors in this study were Robson stage (P = 0.03) and nuclear grade (P = 0.0003). DNA ploidy did not correlate with outcome (P > 0.05). We conclude that cellular proliferation, as measured by SPF analysis, is a significant predictor of outcome for renal cell carcinoma. Although the proliferating cell nuclear antigen index correlated with SPF, it did not achieve statistical significance as a prognostic indicator.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalModern Pathology
Volume8
Issue number1
StatePublished - 1995
Externally publishedYes

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Proliferating Cell Nuclear Antigen
Renal Cell Carcinoma
Cell Proliferation
DNA
Aneuploidy
Ploidies
Nephrectomy
Diploidy
Neoplasms

Keywords

  • cellular proliferation
  • DNA ploidy
  • grade
  • renal cell carcinoma
  • survival

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Prognostic significance of cellular proliferation in renal cell carcinoma : A comparison of synthesis-phase fraction and proliferating cell nuclear antigen index. / Grignon, David; Abdel-Malak, M.; Mertens, W.; Koster, J.; Keeney, M.; Sakr, W.; Shepherd, R. R.

In: Modern Pathology, Vol. 8, No. 1, 1995, p. 18-24.

Research output: Contribution to journalArticle

Grignon, David ; Abdel-Malak, M. ; Mertens, W. ; Koster, J. ; Keeney, M. ; Sakr, W. ; Shepherd, R. R. / Prognostic significance of cellular proliferation in renal cell carcinoma : A comparison of synthesis-phase fraction and proliferating cell nuclear antigen index. In: Modern Pathology. 1995 ; Vol. 8, No. 1. pp. 18-24.
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