Prognostic significance of proliferative and apoptotic fractions in low grade follicle center cell-derived non-Hodgkin's lymphomas

Magdalena Czader, Joanna Mazur, Mikael Pettersson, Jan Liliemark, Mats Strömberg, Birger Christensson, Bernard Tribukait, Gert Auer, Åke Öst, Anna Porwit

Research output: Contribution to journalArticle

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Abstract

BACKGROUND. The biologic parameters, DNA ploidy and proliferative activity, have been suggested as prognostic factors in non-Hodgkin's lymphoma (NHL). However, reports on the prognostic importance of these factors in follicle center cell-derived (FCC) centroblastic/centrocytic (CB/CC) NHL patients with long follow-up are scarce. METHODS. Apoptotic fractions were quantified in 60 patients with CB/CC NHL by in situ labeling of DNA strand breaks in nuclei [TdT-mediated dUTP/dATP in situ 3'OH-end labeling (TUNEL)]. The findings were related to S-phase and MIB-1 counts, DNA ploidy, and clinical outcome. RESULTS. In CB/CC NHL, the percentages of proliferating and apoptotic cells were lower than in reactive germinal centers (GC; P <0.05; mean, 0.188 vs. 3.263% and 19.05 vs. 69.4% for TUNEL and MIB-1 positive cells in CB/CC and GC, respectively). Significantly higher percentages of MIB-1 and TUNEL positive cells were observed in patients with complete remission when compared with the partial remission/no response group (P <0.01). The size of proliferative and apoptotic fractions did not correlate with the overall survival of the patients. However, follicular and diffuse growth pattern, elevated serum lactic dehydrogenase, advanced stage, and age indicated a lower probability of 5- and 10-year survival. CONCLUSIONS. The investigation of proliferative and apoptotic fractions in FCC lymphomas may help to define groups of patients who would benefit from aggressive, high dose therapy protocols and patients to whom less aggressive strategies can be applied safely.

Original languageEnglish (US)
Pages (from-to)1180-1188
Number of pages9
JournalCancer
Volume77
Issue number6
DOIs
StatePublished - Mar 15 1996
Externally publishedYes

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Non-Hodgkin's Lymphoma
In Situ Nick-End Labeling
Ploidies
DNA Breaks
Germinal Center
Survival
DNA
S Phase
Lymphoma
Oxidoreductases
Milk
Growth
Serum

Keywords

  • apoptosis
  • centroblastic- centrocytic
  • DNA ploidy
  • non-Hodgkin's lymphoma
  • proliferation
  • S-phase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Prognostic significance of proliferative and apoptotic fractions in low grade follicle center cell-derived non-Hodgkin's lymphomas. / Czader, Magdalena; Mazur, Joanna; Pettersson, Mikael; Liliemark, Jan; Strömberg, Mats; Christensson, Birger; Tribukait, Bernard; Auer, Gert; Öst, Åke; Porwit, Anna.

In: Cancer, Vol. 77, No. 6, 15.03.1996, p. 1180-1188.

Research output: Contribution to journalArticle

Czader, M, Mazur, J, Pettersson, M, Liliemark, J, Strömberg, M, Christensson, B, Tribukait, B, Auer, G, Öst, Å & Porwit, A 1996, 'Prognostic significance of proliferative and apoptotic fractions in low grade follicle center cell-derived non-Hodgkin's lymphomas', Cancer, vol. 77, no. 6, pp. 1180-1188. https://doi.org/10.1002/(SICI)1097-0142(19960315)77:6<1180::AID-CNCR26>3.0.CO;2-X
Czader, Magdalena ; Mazur, Joanna ; Pettersson, Mikael ; Liliemark, Jan ; Strömberg, Mats ; Christensson, Birger ; Tribukait, Bernard ; Auer, Gert ; Öst, Åke ; Porwit, Anna. / Prognostic significance of proliferative and apoptotic fractions in low grade follicle center cell-derived non-Hodgkin's lymphomas. In: Cancer. 1996 ; Vol. 77, No. 6. pp. 1180-1188.
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abstract = "BACKGROUND. The biologic parameters, DNA ploidy and proliferative activity, have been suggested as prognostic factors in non-Hodgkin's lymphoma (NHL). However, reports on the prognostic importance of these factors in follicle center cell-derived (FCC) centroblastic/centrocytic (CB/CC) NHL patients with long follow-up are scarce. METHODS. Apoptotic fractions were quantified in 60 patients with CB/CC NHL by in situ labeling of DNA strand breaks in nuclei [TdT-mediated dUTP/dATP in situ 3'OH-end labeling (TUNEL)]. The findings were related to S-phase and MIB-1 counts, DNA ploidy, and clinical outcome. RESULTS. In CB/CC NHL, the percentages of proliferating and apoptotic cells were lower than in reactive germinal centers (GC; P <0.05; mean, 0.188 vs. 3.263{\%} and 19.05 vs. 69.4{\%} for TUNEL and MIB-1 positive cells in CB/CC and GC, respectively). Significantly higher percentages of MIB-1 and TUNEL positive cells were observed in patients with complete remission when compared with the partial remission/no response group (P <0.01). The size of proliferative and apoptotic fractions did not correlate with the overall survival of the patients. However, follicular and diffuse growth pattern, elevated serum lactic dehydrogenase, advanced stage, and age indicated a lower probability of 5- and 10-year survival. CONCLUSIONS. The investigation of proliferative and apoptotic fractions in FCC lymphomas may help to define groups of patients who would benefit from aggressive, high dose therapy protocols and patients to whom less aggressive strategies can be applied safely.",
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T1 - Prognostic significance of proliferative and apoptotic fractions in low grade follicle center cell-derived non-Hodgkin's lymphomas

AU - Czader, Magdalena

AU - Mazur, Joanna

AU - Pettersson, Mikael

AU - Liliemark, Jan

AU - Strömberg, Mats

AU - Christensson, Birger

AU - Tribukait, Bernard

AU - Auer, Gert

AU - Öst, Åke

AU - Porwit, Anna

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N2 - BACKGROUND. The biologic parameters, DNA ploidy and proliferative activity, have been suggested as prognostic factors in non-Hodgkin's lymphoma (NHL). However, reports on the prognostic importance of these factors in follicle center cell-derived (FCC) centroblastic/centrocytic (CB/CC) NHL patients with long follow-up are scarce. METHODS. Apoptotic fractions were quantified in 60 patients with CB/CC NHL by in situ labeling of DNA strand breaks in nuclei [TdT-mediated dUTP/dATP in situ 3'OH-end labeling (TUNEL)]. The findings were related to S-phase and MIB-1 counts, DNA ploidy, and clinical outcome. RESULTS. In CB/CC NHL, the percentages of proliferating and apoptotic cells were lower than in reactive germinal centers (GC; P <0.05; mean, 0.188 vs. 3.263% and 19.05 vs. 69.4% for TUNEL and MIB-1 positive cells in CB/CC and GC, respectively). Significantly higher percentages of MIB-1 and TUNEL positive cells were observed in patients with complete remission when compared with the partial remission/no response group (P <0.01). The size of proliferative and apoptotic fractions did not correlate with the overall survival of the patients. However, follicular and diffuse growth pattern, elevated serum lactic dehydrogenase, advanced stage, and age indicated a lower probability of 5- and 10-year survival. CONCLUSIONS. The investigation of proliferative and apoptotic fractions in FCC lymphomas may help to define groups of patients who would benefit from aggressive, high dose therapy protocols and patients to whom less aggressive strategies can be applied safely.

AB - BACKGROUND. The biologic parameters, DNA ploidy and proliferative activity, have been suggested as prognostic factors in non-Hodgkin's lymphoma (NHL). However, reports on the prognostic importance of these factors in follicle center cell-derived (FCC) centroblastic/centrocytic (CB/CC) NHL patients with long follow-up are scarce. METHODS. Apoptotic fractions were quantified in 60 patients with CB/CC NHL by in situ labeling of DNA strand breaks in nuclei [TdT-mediated dUTP/dATP in situ 3'OH-end labeling (TUNEL)]. The findings were related to S-phase and MIB-1 counts, DNA ploidy, and clinical outcome. RESULTS. In CB/CC NHL, the percentages of proliferating and apoptotic cells were lower than in reactive germinal centers (GC; P <0.05; mean, 0.188 vs. 3.263% and 19.05 vs. 69.4% for TUNEL and MIB-1 positive cells in CB/CC and GC, respectively). Significantly higher percentages of MIB-1 and TUNEL positive cells were observed in patients with complete remission when compared with the partial remission/no response group (P <0.01). The size of proliferative and apoptotic fractions did not correlate with the overall survival of the patients. However, follicular and diffuse growth pattern, elevated serum lactic dehydrogenase, advanced stage, and age indicated a lower probability of 5- and 10-year survival. CONCLUSIONS. The investigation of proliferative and apoptotic fractions in FCC lymphomas may help to define groups of patients who would benefit from aggressive, high dose therapy protocols and patients to whom less aggressive strategies can be applied safely.

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