Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice

M. E. Steinhelper, N. A. Lanson, K. P. Dresdner, J. B. Delcarpio, A. L. Wit, W. C. Claycomb, Loren Field

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Transgenic mice expressing atrial natriuretic factor-SV40 T-antigen fusion genes (ANF-TAG) developed unilateral right atrial tumors composed of differentiated dividing cardiomyocytes. The atrial tumors could be propagated as transplantable tumor lineages in syngeneic animals. Cardiomyocytes derived from ANF-TAG atrial tumors did not proliferate in tissue culture. However, cardiomyocytes derived from the transplantable tumor lines proliferated in culture, and these proliferating cardiomyocytes could be passaged in culture and recovered from frozen stocks. Cardiomyocytes from either tumor source were highly differentiated as determined by diverse functional and structural criteria. The cells continued to express numerous cardiac-specific proteins and retained ultrastructural features characteristic of cardiomyocytes including well-formed myofibrils, transverse tubules, and intercalated disks. In addition, the cultured cells displayed spontaneous electrical and contractile activities. These atrial tumor cardiomyocytes are a novel experimental resource for the identification of genes regulating the cardiomyocyte cell cycle.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume259
Issue number6 28-6
StatePublished - 1990

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Cardiac Myocytes
Transgenic Mice
Neoplasms
Polyomavirus Transforming Antigens
Gene Fusion
Atrial Natriuretic Factor
Myofibrils
Cultured Cells
Cell Cycle
Genes
Proteins

Keywords

  • cardiac muscle cells
  • cell culture
  • heart tumors
  • SV40 T-antigen

ASJC Scopus subject areas

  • Physiology

Cite this

Steinhelper, M. E., Lanson, N. A., Dresdner, K. P., Delcarpio, J. B., Wit, A. L., Claycomb, W. C., & Field, L. (1990). Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice. American Journal of Physiology - Heart and Circulatory Physiology, 259(6 28-6).

Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice. / Steinhelper, M. E.; Lanson, N. A.; Dresdner, K. P.; Delcarpio, J. B.; Wit, A. L.; Claycomb, W. C.; Field, Loren.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 259, No. 6 28-6, 1990.

Research output: Contribution to journalArticle

Steinhelper, M. E. ; Lanson, N. A. ; Dresdner, K. P. ; Delcarpio, J. B. ; Wit, A. L. ; Claycomb, W. C. ; Field, Loren. / Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice. In: American Journal of Physiology - Heart and Circulatory Physiology. 1990 ; Vol. 259, No. 6 28-6.
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AU - Lanson, N. A.

AU - Dresdner, K. P.

AU - Delcarpio, J. B.

AU - Wit, A. L.

AU - Claycomb, W. C.

AU - Field, Loren

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