Proliferation of human hematopoietic bone marrow cells in simulated microgravity

P. A. Plett, S. M. Frankovitz, Rafat Abonour, Christie Orschell

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Expansion and/or maintenance of hematopoietic stem cell (HSC) potential following in vitro culture remains a major obstacle in stem cell biology and bone marrow (BM) transplantation. Several studies suggest that culture of mammalian cells in microgravity (μ-g) may reduce proliferation and differentiation of these cells. We investigated the application of these findings to the field of stem cell biology in the hopes of expanding HSC with minimal loss of hematopoietic function. To this end, BM CD34+ cells were cultured for 4-6 d in rotating wall vessels for simulation of μ-g, and assessed for expansion, cell cycle activation, apoptosis, and hematopoietic potential. While CD34+ cells cultured in normal gravity (1-g) proliferated up to threefold by day 4-6, cells cultured in μ-g did not increase in number. As a possible explanation for this, cells cultured in simulated μ-g were found to exit G0/G1 phase of cell cycle at a slower rate than 1-g controls. When assayed for primitive hematopoietic potential in secondary conventional 1-g long-term cultures, cells from initial μ-g cultures produced greater numbers of cells and progenitors, and for a longer period of time, than cultures initiated with 1-g control cells. Similar low levels of apoptosis and adhesion molecule phenotype in μ-g and 1-g-cultured cells suggested similar growth patterns in the two settings. These data begin to elucidate the effects of μ-g on proliferation of human hematopoietic cells and may be potentially beneficial to the fields of stem cell biology and somatic gene therapy.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume37
Issue number2
DOIs
StatePublished - 2001

Fingerprint

Weightlessness
Microgravity
Stem cells
Bone Marrow Cells
Cytology
Cultured Cells
Bone
Stem Cells
Cell culture
Cells
Cell Biology
Hematopoietic Stem Cells
Cell Cycle
Cell Culture Techniques
Apoptosis
Cell Cycle Resting Phase
G1 Phase
Gravitation
Gene therapy
Bone Marrow Transplantation

Keywords

  • Bone marrow
  • CD34
  • Cell culture
  • Cell cycle
  • Ex vivo expansion
  • Stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Proliferation of human hematopoietic bone marrow cells in simulated microgravity. / Plett, P. A.; Frankovitz, S. M.; Abonour, Rafat; Orschell, Christie.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 37, No. 2, 2001, p. 73-78.

Research output: Contribution to journalArticle

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