Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks

C. H. Lee, N. L. Bauer, M. M. Shaw, M. M. Durkin, M. S. Bartlett, S. F. Queener, J. W. Smith

Research output: Contribution to journalComment/debate

31 Scopus citations

Abstract

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

Original languageEnglish (US)
Pages (from-to)1659-1662
Number of pages4
JournalJournal of clinical microbiology
Volume31
Issue number6
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Microbiology (medical)

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    Lee, C. H., Bauer, N. L., Shaw, M. M., Durkin, M. M., Bartlett, M. S., Queener, S. F., & Smith, J. W. (1993). Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. Journal of clinical microbiology, 31(6), 1659-1662.