Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks

Chao-Hung Lee, N. L. Bauer, M. M. Shaw, M. M. Durkin, M. S. Bartlett, Sherry Queener, J. W. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

Original languageEnglish
Pages (from-to)1659-1662
Number of pages4
JournalJournal of Clinical Microbiology
Volume31
Issue number6
StatePublished - 1993

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Pneumocystis carinii
Trophozoites
Fibroblasts
Antigens
Lung
DNA

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Lee, C-H., Bauer, N. L., Shaw, M. M., Durkin, M. M., Bartlett, M. S., Queener, S., & Smith, J. W. (1993). Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. Journal of Clinical Microbiology, 31(6), 1659-1662.

Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. / Lee, Chao-Hung; Bauer, N. L.; Shaw, M. M.; Durkin, M. M.; Bartlett, M. S.; Queener, Sherry; Smith, J. W.

In: Journal of Clinical Microbiology, Vol. 31, No. 6, 1993, p. 1659-1662.

Research output: Contribution to journalArticle

Lee, C-H, Bauer, NL, Shaw, MM, Durkin, MM, Bartlett, MS, Queener, S & Smith, JW 1993, 'Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks', Journal of Clinical Microbiology, vol. 31, no. 6, pp. 1659-1662.
Lee, Chao-Hung ; Bauer, N. L. ; Shaw, M. M. ; Durkin, M. M. ; Bartlett, M. S. ; Queener, Sherry ; Smith, J. W. / Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 6. pp. 1659-1662.
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