Proliferative capacity and osteogenic potential of novel dura mater stem cells on poly-lactic-co-glycolic acid

Caren Petrie, Sunil Tholpady, Roy Ogle, Edward Botchwey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The rational design of biomimetic structures for the regeneration of damaged or missing tissue is a fundamental principle of tissue engineering. Multiple variables must be optimized, ranging from the scaffold type to the selection and properties of implanted cell(s). In this study, the osteogenic potential of a novel stem cell was analyzed on biodegradable poly(lactic-co-glycolic acid) (PLGA) biomaterials as a step toward creating new cell-materials constructs for bony regeneration. Dura mater stem cells (DSCs), isolated from rat dura mater, were evaluated and compared to bone marrow stem cells (BMSCs) for proliferative and differentiative properties in vitro. Experiments were carried out on both tissue culture plastic (TCP) and 2D planar films of PLGA. Proliferation of DSCs on both TCP and PLGA films increased over 21 days. Positive fold inductions in all five bone marker genes were observed at days 7, 14, 21 in all experimental samples compared with day 0 controls. DSCs demonstrated greater cell coverage and enhanced matrix staining on 2D PLGA films when compared with BMSCs. These cells can be isolated and expanded in culture and can subsequently attach, proliferate, and differentiate on both TCP and PLGA films to a greater extent than BMSCs. This suggests that DSCs are promising for cell-based bone tissue engineering therapies, particularly those applications involving regeneration of cranial bones.

Original languageEnglish (US)
Pages (from-to)61-71
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume85
Issue number1
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Stem cells
Bone
Acids
Tissue culture
Plastics
Tissue engineering
Milk
polylactic acid-polyglycolic acid copolymer
Biomimetics
Biocompatible Materials
Biomaterials
Scaffolds
Rats
Genes
Tissue

Keywords

  • Biomimetic material
  • Bone regeneration
  • Bone tissue engineering
  • Dura mater
  • Plasticity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Proliferative capacity and osteogenic potential of novel dura mater stem cells on poly-lactic-co-glycolic acid. / Petrie, Caren; Tholpady, Sunil; Ogle, Roy; Botchwey, Edward.

In: Journal of Biomedical Materials Research - Part A, Vol. 85, No. 1, 04.2008, p. 61-71.

Research output: Contribution to journalArticle

Petrie, Caren ; Tholpady, Sunil ; Ogle, Roy ; Botchwey, Edward. / Proliferative capacity and osteogenic potential of novel dura mater stem cells on poly-lactic-co-glycolic acid. In: Journal of Biomedical Materials Research - Part A. 2008 ; Vol. 85, No. 1. pp. 61-71.
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