Prolonged membrane potential depolarization in cingulate pyramidal cells after digit amputation in adult rats

Min F. Wu, Zhiping P. Pang, Min Zhuo, Z. C. Xu

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The anterior cingulate cortex (ACC) plays an important role in higher brain functions including learning, memory, and persistent pain. Long-term potentiation of excitatory synaptic transmission has been observed in the ACC after digit amputation, which might contribute to plastic changes associated with the phantom pain. Here we report a long-lasting membrane potential depolarization in ACC neurons of adult rats after digit amputation in vivo. Shortly after digit amputation of the hind paw, the membrane potential of intracellularly recorded ACC neurons quickly depolarized from ∼-70 mV to ∼-15 mV and then slowly repolarized. The duration of this amputation-induced depolarization was about 40 min. Intracellular staining revealed that these neurons were pyramidal neurons in the ACC. The depolarization is activity-dependent, since peripheral application of lidocaine significantly reduced it. Furthermore, the depolarization was significantly reduced by a NMDA receptor antagonist MK-801. Our results provide direct in vivo electrophysiological evidence that ACC pyramidal cells undergo rapid and prolonged depolarization after digit amputation, and the amputation-induced depolarization in ACC neurons might be associated with the synaptic mechanisms for phantom pain.

Original languageEnglish
Article number23
JournalMolecular Pain
Volume1
DOIs
StatePublished - Aug 19 2005

Fingerprint

Pyramidal Cells
Gyrus Cinguli
Amputation
Membrane Potentials
Phantom Limb
Neurons
Dizocilpine Maleate
Long-Term Potentiation
Lidocaine
N-Methyl-D-Aspartate Receptors
Synaptic Transmission
Learning
Staining and Labeling
Pain
Brain

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Prolonged membrane potential depolarization in cingulate pyramidal cells after digit amputation in adult rats. / Wu, Min F.; Pang, Zhiping P.; Zhuo, Min; Xu, Z. C.

In: Molecular Pain, Vol. 1, 23, 19.08.2005.

Research output: Contribution to journalArticle

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