Proper Organization of Microtubule Minus Ends Is Needed for Midzone Stability and Cytokinesis

Shang Cai, Lesley N. Weaver, Stephanie C. Ems-McClung, Claire Walczak

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Successful cytokinesis is critical for maintaining genome stability [1, 2] and requires the assembly of a robust central spindle to specify the cleavage furrow position [3], to prevent separated chromosomes from coming back together [4], and to contribute to midbody abscission [5, 6]. A proper central spindle is assembled and maintained by a number of microtubule-associated and molecular motor proteins that sort microtubules into bundles with their plus ends overlapping at the center [1, 2]. The mechanisms by which different factors organize the central spindle microtubules remain unclear. We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequency of polyploid cells, which resulted from a failure in cytokinesis. In addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microtubule minus ends, as well as mislocalization of several midbody-associated proteins. Biochemical analysis showed that both human HSET and Xenopus XCTK2 cofractionated with the γ-tubulin ring complexes on sucrose gradients and that XCTK2 associated with γ-tubulin and Xgrip109 by immunoprecipitation. Our data reveal the novel finding that a minus-end-directed motor contributes to the organization and stability of the central spindle, which is needed for proper cytokinesis.

Original languageEnglish
Pages (from-to)880-885
Number of pages6
JournalCurrent Biology
Volume20
Issue number9
DOIs
StatePublished - May 11 2010

Fingerprint

Cytokinesis
cytokinesis
Tubulin
Microtubules
microtubules
Molecular Motor Proteins
Kinesin
Chromosomes
Sucrose
tubulin
Genes
molecular motor proteins
kinesin
Polyploidy
Genomic Instability
abscission
Xenopus
furrows
Proteins
Immunoprecipitation

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Proper Organization of Microtubule Minus Ends Is Needed for Midzone Stability and Cytokinesis. / Cai, Shang; Weaver, Lesley N.; Ems-McClung, Stephanie C.; Walczak, Claire.

In: Current Biology, Vol. 20, No. 9, 11.05.2010, p. 880-885.

Research output: Contribution to journalArticle

Cai, Shang ; Weaver, Lesley N. ; Ems-McClung, Stephanie C. ; Walczak, Claire. / Proper Organization of Microtubule Minus Ends Is Needed for Midzone Stability and Cytokinesis. In: Current Biology. 2010 ; Vol. 20, No. 9. pp. 880-885.
@article{9dcfab7c748143a2ad295e49e80d68a8,
title = "Proper Organization of Microtubule Minus Ends Is Needed for Midzone Stability and Cytokinesis",
abstract = "Successful cytokinesis is critical for maintaining genome stability [1, 2] and requires the assembly of a robust central spindle to specify the cleavage furrow position [3], to prevent separated chromosomes from coming back together [4], and to contribute to midbody abscission [5, 6]. A proper central spindle is assembled and maintained by a number of microtubule-associated and molecular motor proteins that sort microtubules into bundles with their plus ends overlapping at the center [1, 2]. The mechanisms by which different factors organize the central spindle microtubules remain unclear. We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequency of polyploid cells, which resulted from a failure in cytokinesis. In addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microtubule minus ends, as well as mislocalization of several midbody-associated proteins. Biochemical analysis showed that both human HSET and Xenopus XCTK2 cofractionated with the γ-tubulin ring complexes on sucrose gradients and that XCTK2 associated with γ-tubulin and Xgrip109 by immunoprecipitation. Our data reveal the novel finding that a minus-end-directed motor contributes to the organization and stability of the central spindle, which is needed for proper cytokinesis.",
keywords = "CELLBIO",
author = "Shang Cai and Weaver, {Lesley N.} and Ems-McClung, {Stephanie C.} and Claire Walczak",
year = "2010",
month = "5",
day = "11",
doi = "10.1016/j.cub.2010.03.067",
language = "English",
volume = "20",
pages = "880--885",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "9",

}

TY - JOUR

T1 - Proper Organization of Microtubule Minus Ends Is Needed for Midzone Stability and Cytokinesis

AU - Cai, Shang

AU - Weaver, Lesley N.

AU - Ems-McClung, Stephanie C.

AU - Walczak, Claire

PY - 2010/5/11

Y1 - 2010/5/11

N2 - Successful cytokinesis is critical for maintaining genome stability [1, 2] and requires the assembly of a robust central spindle to specify the cleavage furrow position [3], to prevent separated chromosomes from coming back together [4], and to contribute to midbody abscission [5, 6]. A proper central spindle is assembled and maintained by a number of microtubule-associated and molecular motor proteins that sort microtubules into bundles with their plus ends overlapping at the center [1, 2]. The mechanisms by which different factors organize the central spindle microtubules remain unclear. We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequency of polyploid cells, which resulted from a failure in cytokinesis. In addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microtubule minus ends, as well as mislocalization of several midbody-associated proteins. Biochemical analysis showed that both human HSET and Xenopus XCTK2 cofractionated with the γ-tubulin ring complexes on sucrose gradients and that XCTK2 associated with γ-tubulin and Xgrip109 by immunoprecipitation. Our data reveal the novel finding that a minus-end-directed motor contributes to the organization and stability of the central spindle, which is needed for proper cytokinesis.

AB - Successful cytokinesis is critical for maintaining genome stability [1, 2] and requires the assembly of a robust central spindle to specify the cleavage furrow position [3], to prevent separated chromosomes from coming back together [4], and to contribute to midbody abscission [5, 6]. A proper central spindle is assembled and maintained by a number of microtubule-associated and molecular motor proteins that sort microtubules into bundles with their plus ends overlapping at the center [1, 2]. The mechanisms by which different factors organize the central spindle microtubules remain unclear. We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequency of polyploid cells, which resulted from a failure in cytokinesis. In addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microtubule minus ends, as well as mislocalization of several midbody-associated proteins. Biochemical analysis showed that both human HSET and Xenopus XCTK2 cofractionated with the γ-tubulin ring complexes on sucrose gradients and that XCTK2 associated with γ-tubulin and Xgrip109 by immunoprecipitation. Our data reveal the novel finding that a minus-end-directed motor contributes to the organization and stability of the central spindle, which is needed for proper cytokinesis.

KW - CELLBIO

UR - http://www.scopus.com/inward/record.url?scp=77953133964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953133964&partnerID=8YFLogxK

U2 - 10.1016/j.cub.2010.03.067

DO - 10.1016/j.cub.2010.03.067

M3 - Article

VL - 20

SP - 880

EP - 885

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 9

ER -