Proposed molten globule intermediates in fd phage penetration and assembly

A. Keith Dunker, Lars D. Ensign, Gregory E. Arnold, Linda M. Roberts

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The fd filamentous phage can be contracted to short rods called I-forms and to spheroidal particles called S-forms. The conversions from fd→I-forms→S-forms were previously suggested to mimic steps in fd penetration. The same conversions, in reverse order, were suggested to mimic steps in fd assembly. The I-forms and S-forms bind the hydrophobic probe, 1-anilino-napthalene-8-sulionate (ANS); under the same conditions, fd binds this probe very poorly. Rigidly packed side chains in fd and nonrigidly packed side chains in I-forms and S-forms would explain the differences in ANS binding. A compilation of the properties of I-forms and S-forms indicate that: (i) they have compact structures; (ii) they have secondary structures of the same type as native phage; (iii) they have non-native morphologies; and (iv) they may have nonrigid side chain packing. These are the properties of molten globules.

Original languageEnglish (US)
Pages (from-to)275-278
Number of pages4
JournalFEBS Letters
Volume292
Issue number1-2
DOIs
StatePublished - Nov 4 1991

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Keywords

  • Filamentous phage assembly
  • Molten globule intermediate

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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