Prostaglandin E acts at two levels to enhance colony formation in vitro by erythroid (BFU-E) progenitor cells

L. Lu, Louis Pelus, W. Piacibello, M. A S Moore, W. Hu, Hal Broxmeyer

Research output: Contribution to journalArticle

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Abstract

The prostaglandin E (PGE) enhancement of erythroid colony formation by human bone marrow erythroid progenitor cells (BFU-E) is mediated by a T8+ subset of lymphocytes. Medium was conditioned by bone marrow and blood T-lymphocytes and T-lymphocyte subsets (T8+, T8-, T4+, and T4- cells) in the absence or presence of PGE1 in order to determine if the cells could release a cell-free source of erythroid colony enhancing activity and what the conditions for this release would be. The T-lymphocyte conditioned medium was assayed for its effects on erythroid colony formation by nonadherent low-density T-lymphocyte depleted (NALT-) bone marrow cells plated in the presence of erythropoietin, hemin, phytohemagglutinin-stimulated leukocyte conditioned medium, or medium conditioned by 5637 cells, in the absence or presence of PGE1 and in the presence or absence of serum. PGE1 induced the release of an erythroid colony enhancing activity from the T8+ and T4-, but not from the T8- and T4+ subsets of lymphocytes, but this cell-free source of activity was only apparent if it was tested for colony formation in the presence of added PGE1. The release and action of the PGE1 induced T-lymphocyte erythroid enhancing activity did not require the presence of serum. Erythroid colony formation by NALT- bone marrow cells was not enhanced by PGE1 alone, by medium conditioned by T-lymphocytes in the absence of PGE1 or by PGE1 plus medium conditioned by T-lymphocytes in the absence of PGE1. The results suggest that the PGE1 enhancement of erythroid colony formation occurs by an apparently synergistic action on non-T-lymphocytes by PGE1 itself and by a factor or factors released from T8+ lymphocytes in response to PGE1.

Original languageEnglish
Pages (from-to)765-771
Number of pages7
JournalExperimental Hematology
Volume15
Issue number7
StatePublished - 1987

Fingerprint

Erythroid Precursor Cells
Alprostadil
Prostaglandins E
Stem Cells
Conditioned Culture Medium
T-Lymphocytes
Bone Marrow Cells
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
In Vitro Techniques
Hemin
T-Lymphocyte Subsets
Phytohemagglutinins
Erythropoietin
Serum
Leukocytes
Bone Marrow

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Prostaglandin E acts at two levels to enhance colony formation in vitro by erythroid (BFU-E) progenitor cells. / Lu, L.; Pelus, Louis; Piacibello, W.; Moore, M. A S; Hu, W.; Broxmeyer, Hal.

In: Experimental Hematology, Vol. 15, No. 7, 1987, p. 765-771.

Research output: Contribution to journalArticle

Lu, L. ; Pelus, Louis ; Piacibello, W. ; Moore, M. A S ; Hu, W. ; Broxmeyer, Hal. / Prostaglandin E acts at two levels to enhance colony formation in vitro by erythroid (BFU-E) progenitor cells. In: Experimental Hematology. 1987 ; Vol. 15, No. 7. pp. 765-771.
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