Abstract
Prostaglandin E1 and E2 (PGE) antagonize the phosphaturic effect of parathyroid hormone (PTH), but do not alter the phosphaturia evoked by adenosine 3',5'-cyclic monophosphate (cAMP) analogues. These findings support the idea that PGE interfere with activation of adenylate cyclase in the renal proximal tubule. We tested this hypothesis in the rabbit renal proximal straight tubule (PST). In the PST, adenylate cyclase was activated by PTH (K(m) = 10-9 M PTH), but not by PGE2, which attenuate the activation of adenylate cyclase by PTH. The inhibition by PGE2 of PTH action was prevented by pertussis toxin, which deactivates the regulatory aggregate, N(i). In the PST, PGE2 also attenuated the activation of adenylate cyclase by cholera toxin. The inhibitory effect of PGE2 was selective; PGE2 did not inhibit activation of ademylate cyclase inglomeruli, but it inhibited the enzyme in proximal convoluted tubules (PCT) and PST. We conclude that PGE2 inhibits adenylate cyclase in rabbit proximal tubule. We propose that this action may, in part, regulate transport function in vivo.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 254 |
| Issue number | 2 |
| State | Published - 1988 |
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ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Physiology
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Prostaglandin E2 is an inhibitor of adenylate cyclase in rabbit proximal tubule. / Dominguez, Jesus; Shuler, F.; Olszowy, M. W.; Brown, T.; Puschett, J. B.
In: American Journal of Physiology - Cell Physiology, Vol. 254, No. 2, 1988.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Prostaglandin E2 is an inhibitor of adenylate cyclase in rabbit proximal tubule
AU - Dominguez, Jesus
AU - Shuler, F.
AU - Olszowy, M. W.
AU - Brown, T.
AU - Puschett, J. B.
PY - 1988
Y1 - 1988
N2 - Prostaglandin E1 and E2 (PGE) antagonize the phosphaturic effect of parathyroid hormone (PTH), but do not alter the phosphaturia evoked by adenosine 3',5'-cyclic monophosphate (cAMP) analogues. These findings support the idea that PGE interfere with activation of adenylate cyclase in the renal proximal tubule. We tested this hypothesis in the rabbit renal proximal straight tubule (PST). In the PST, adenylate cyclase was activated by PTH (K(m) = 10-9 M PTH), but not by PGE2, which attenuate the activation of adenylate cyclase by PTH. The inhibition by PGE2 of PTH action was prevented by pertussis toxin, which deactivates the regulatory aggregate, N(i). In the PST, PGE2 also attenuated the activation of adenylate cyclase by cholera toxin. The inhibitory effect of PGE2 was selective; PGE2 did not inhibit activation of ademylate cyclase inglomeruli, but it inhibited the enzyme in proximal convoluted tubules (PCT) and PST. We conclude that PGE2 inhibits adenylate cyclase in rabbit proximal tubule. We propose that this action may, in part, regulate transport function in vivo.
AB - Prostaglandin E1 and E2 (PGE) antagonize the phosphaturic effect of parathyroid hormone (PTH), but do not alter the phosphaturia evoked by adenosine 3',5'-cyclic monophosphate (cAMP) analogues. These findings support the idea that PGE interfere with activation of adenylate cyclase in the renal proximal tubule. We tested this hypothesis in the rabbit renal proximal straight tubule (PST). In the PST, adenylate cyclase was activated by PTH (K(m) = 10-9 M PTH), but not by PGE2, which attenuate the activation of adenylate cyclase by PTH. The inhibition by PGE2 of PTH action was prevented by pertussis toxin, which deactivates the regulatory aggregate, N(i). In the PST, PGE2 also attenuated the activation of adenylate cyclase by cholera toxin. The inhibitory effect of PGE2 was selective; PGE2 did not inhibit activation of ademylate cyclase inglomeruli, but it inhibited the enzyme in proximal convoluted tubules (PCT) and PST. We conclude that PGE2 inhibits adenylate cyclase in rabbit proximal tubule. We propose that this action may, in part, regulate transport function in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=18244423608&partnerID=8YFLogxK
M3 - Article
C2 - 3162352
AN - SCOPUS:18244423608
VL - 254
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0193-1857
IS - 2
ER -