Prostaglandin E2 is an inhibitor of adenylate cyclase in rabbit proximal tubule

Jesus Dominguez, F. Shuler, M. W. Olszowy, T. Brown, J. B. Puschett

Research output: Contribution to journalArticle

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Abstract

Prostaglandin E1 and E2 (PGE) antagonize the phosphaturic effect of parathyroid hormone (PTH), but do not alter the phosphaturia evoked by adenosine 3',5'-cyclic monophosphate (cAMP) analogues. These findings support the idea that PGE interfere with activation of adenylate cyclase in the renal proximal tubule. We tested this hypothesis in the rabbit renal proximal straight tubule (PST). In the PST, adenylate cyclase was activated by PTH (K(m) = 10-9 M PTH), but not by PGE2, which attenuate the activation of adenylate cyclase by PTH. The inhibition by PGE2 of PTH action was prevented by pertussis toxin, which deactivates the regulatory aggregate, N(i). In the PST, PGE2 also attenuated the activation of adenylate cyclase by cholera toxin. The inhibitory effect of PGE2 was selective; PGE2 did not inhibit activation of ademylate cyclase inglomeruli, but it inhibited the enzyme in proximal convoluted tubules (PCT) and PST. We conclude that PGE2 inhibits adenylate cyclase in rabbit proximal tubule. We propose that this action may, in part, regulate transport function in vivo.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume254
Issue number2
StatePublished - 1988

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Dinoprostone
Adenylyl Cyclases
Rabbits
Parathyroid Hormone
Chemical activation
Proximal Kidney Tubule
Alprostadil
Adenylate Cyclase Toxin
Familial Hypophosphatemia
Adenylyl Cyclase Inhibitors
Cholera Toxin
Pertussis Toxin
Cyclic AMP
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Prostaglandin E2 is an inhibitor of adenylate cyclase in rabbit proximal tubule. / Dominguez, Jesus; Shuler, F.; Olszowy, M. W.; Brown, T.; Puschett, J. B.

In: American Journal of Physiology - Cell Physiology, Vol. 254, No. 2, 1988.

Research output: Contribution to journalArticle

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AU - Shuler, F.

AU - Olszowy, M. W.

AU - Brown, T.

AU - Puschett, J. B.

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