Protective effect of L-arginine on hypercholesterolemia-enhanced renal ischemic injury

Silvia Campos-Bilderback, Maria Ori, Egídio Lima Dórea, Antonio Carlos Seguro

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effects of hypercholesterolemia on ischemic renal failure were evaluated in rats subjected to 60 min of left renal artery clamping and contralateral nephrectomy. One group of rats (HC) was kept on a cholesterol- supplemented diet for 3 weeks before renal injury and compared to a group fed a regular diet (ND). Two days after renal ischemia, inulin clearance (C(in), ml/min per 100 g BW) was lower in HC-rats (0.033 ± 0.011) than in ND-rats (0.227 ± 0.037; P < 0.01). indicating that hypercholesterolemia potentiated renal ischemic injury. Twenty-one days after renal ischemia the C(in) of HC- rats did not differ from ND-rats, suggesting that hypercholesterolemia did not limit late recovery. Since nitric oxide production is impaired in HC, L- arginine (50 mg/kg BW i.v.) was administered immediately after ischemia. Two days after ischemia, L-arg did not protect ND-rats from ischemia, while the C(in) and renal blood flow were higher in L-arg-treated HC rats than in untreated HC rats (C(in) =0.125 ± 0.013 rats vs. 0.033 ± 0.011; P < 0.001) (RBF = 3.96 ± 0.64 vs. 2.40 ± 0.20 ml/min per 100 g BW; P < 0.05), indicating that L-arg protects HC rats from renal ischemia. The administration of D-arginine to ND rats induced a significant decrease of the C(in) and a significant increase of FE H 2O, FE Na and FE K compared to the L-arginine and not treated groups. Cultures of inner medullary collecting duct cells from ND rats were resistant to 24-h hypoxia. In contrast, IMCD cell cultures from HC rats showed higher LDH release after 24-h hypoxia than normoxic cells (69.2 ± 3.4 vs. 30.9 ± 3.6%, P < 0.001); 1 mM L-arg added to the medium attenuated LDH release (44.3 ± 2.4%, P < 0.01). These data demonstrate that HC predisposes renal tubular cells to hypoxic injury and L- arg protects cells of HC.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalAtherosclerosis
Volume143
Issue number2
DOIs
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Hypercholesterolemia
Arginine
Kidney
Wounds and Injuries
Ischemia
Diet
Cell Hypoxia
Inulin
Renal Circulation
Renal Artery
Nephrectomy
Constriction
Renal Insufficiency
Nitric Oxide
Cell Culture Techniques
Cholesterol

Keywords

  • Acute renal failure
  • Hypercholesterolemia
  • L-arginine
  • Nitric oxide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Protective effect of L-arginine on hypercholesterolemia-enhanced renal ischemic injury. / Campos-Bilderback, Silvia; Ori, Maria; Dórea, Egídio Lima; Seguro, Antonio Carlos.

In: Atherosclerosis, Vol. 143, No. 2, 04.1999, p. 327-334.

Research output: Contribution to journalArticle

Campos-Bilderback, Silvia ; Ori, Maria ; Dórea, Egídio Lima ; Seguro, Antonio Carlos. / Protective effect of L-arginine on hypercholesterolemia-enhanced renal ischemic injury. In: Atherosclerosis. 1999 ; Vol. 143, No. 2. pp. 327-334.
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