Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves

Roza K. Sypniewska, Nancy J. Millenbaugh, Johnathan L. Kiel, Robert V. Blystone, Heather N. Ringham, Patrick A. Mason, Frank Witzmann

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A macrophage assay and proteomic screening were used to investigate the biological activity of soluble factors in the plasma of millimeter wave-exposed rats. NR8383 rat macrophages were incubated for 24 h with 10% plasma from male Sprague-Dawley rats that had been exposed to sham conditions, or exposed to 42 °C environmental heat or 35 GHz millimeter waves at 75 mW/cm2 until core temperature reached 41.0 °C. Two-dimensional polyacrylamide gel electrophoresis, image analysis, and Western blotting were used to analyze approximately 600 protein spots in the cell lysates for changes in protein abundance and levels of 3-nitrotyrosine, a marker of macrophage stimulation. Proteins of interest were identified using peptide mass fingerprinting. Compared to plasma from sham-exposed rats, plasma from environmental heat- or millimeter wave-exposed rats increased the expression of 11 proteins, and levels of 3-nitrotyrosine in seven proteins, in the NR8383 cells. These altered proteins are associated with inflammation, oxidative stress, and energy metabolism. Findings of this study indicate both environmental heat and 35 GHz millimeter wave exposure elicit the release of macrophage-activating mediators into the plasma of rats.

Original languageEnglish
Pages (from-to)656-663
Number of pages8
JournalBioelectromagnetics
Volume31
Issue number8
DOIs
StatePublished - Dec 2010

Fingerprint

Macrophages
Proteins
Hot Temperature
Peptide Mapping
Electrophoresis, Gel, Two-Dimensional
Proteomics
Energy Metabolism
Sprague Dawley Rats
Oxidative Stress
Western Blotting
Inflammation
Temperature
3-nitrotyrosine

Keywords

  • 3-nitrotyrosine
  • Oxidative stress
  • Proteomics
  • Radiofrequency
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Physiology

Cite this

Sypniewska, R. K., Millenbaugh, N. J., Kiel, J. L., Blystone, R. V., Ringham, H. N., Mason, P. A., & Witzmann, F. (2010). Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves. Bioelectromagnetics, 31(8), 656-663. https://doi.org/10.1002/bem.20598

Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves. / Sypniewska, Roza K.; Millenbaugh, Nancy J.; Kiel, Johnathan L.; Blystone, Robert V.; Ringham, Heather N.; Mason, Patrick A.; Witzmann, Frank.

In: Bioelectromagnetics, Vol. 31, No. 8, 12.2010, p. 656-663.

Research output: Contribution to journalArticle

Sypniewska, RK, Millenbaugh, NJ, Kiel, JL, Blystone, RV, Ringham, HN, Mason, PA & Witzmann, F 2010, 'Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves', Bioelectromagnetics, vol. 31, no. 8, pp. 656-663. https://doi.org/10.1002/bem.20598
Sypniewska RK, Millenbaugh NJ, Kiel JL, Blystone RV, Ringham HN, Mason PA et al. Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves. Bioelectromagnetics. 2010 Dec;31(8):656-663. https://doi.org/10.1002/bem.20598
Sypniewska, Roza K. ; Millenbaugh, Nancy J. ; Kiel, Johnathan L. ; Blystone, Robert V. ; Ringham, Heather N. ; Mason, Patrick A. ; Witzmann, Frank. / Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves. In: Bioelectromagnetics. 2010 ; Vol. 31, No. 8. pp. 656-663.
@article{a43591b8061c46b0a66bf57b244289af,
title = "Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves",
abstract = "A macrophage assay and proteomic screening were used to investigate the biological activity of soluble factors in the plasma of millimeter wave-exposed rats. NR8383 rat macrophages were incubated for 24 h with 10{\%} plasma from male Sprague-Dawley rats that had been exposed to sham conditions, or exposed to 42 °C environmental heat or 35 GHz millimeter waves at 75 mW/cm2 until core temperature reached 41.0 °C. Two-dimensional polyacrylamide gel electrophoresis, image analysis, and Western blotting were used to analyze approximately 600 protein spots in the cell lysates for changes in protein abundance and levels of 3-nitrotyrosine, a marker of macrophage stimulation. Proteins of interest were identified using peptide mass fingerprinting. Compared to plasma from sham-exposed rats, plasma from environmental heat- or millimeter wave-exposed rats increased the expression of 11 proteins, and levels of 3-nitrotyrosine in seven proteins, in the NR8383 cells. These altered proteins are associated with inflammation, oxidative stress, and energy metabolism. Findings of this study indicate both environmental heat and 35 GHz millimeter wave exposure elicit the release of macrophage-activating mediators into the plasma of rats.",
keywords = "3-nitrotyrosine, Oxidative stress, Proteomics, Radiofrequency, Two-dimensional gel electrophoresis",
author = "Sypniewska, {Roza K.} and Millenbaugh, {Nancy J.} and Kiel, {Johnathan L.} and Blystone, {Robert V.} and Ringham, {Heather N.} and Mason, {Patrick A.} and Frank Witzmann",
year = "2010",
month = "12",
doi = "10.1002/bem.20598",
language = "English",
volume = "31",
pages = "656--663",
journal = "Bioelectromagnetics",
issn = "0197-8462",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Protein changes in macrophages induced by plasma from rats exposed to 35 GHz millimeter waves

AU - Sypniewska, Roza K.

AU - Millenbaugh, Nancy J.

AU - Kiel, Johnathan L.

AU - Blystone, Robert V.

AU - Ringham, Heather N.

AU - Mason, Patrick A.

AU - Witzmann, Frank

PY - 2010/12

Y1 - 2010/12

N2 - A macrophage assay and proteomic screening were used to investigate the biological activity of soluble factors in the plasma of millimeter wave-exposed rats. NR8383 rat macrophages were incubated for 24 h with 10% plasma from male Sprague-Dawley rats that had been exposed to sham conditions, or exposed to 42 °C environmental heat or 35 GHz millimeter waves at 75 mW/cm2 until core temperature reached 41.0 °C. Two-dimensional polyacrylamide gel electrophoresis, image analysis, and Western blotting were used to analyze approximately 600 protein spots in the cell lysates for changes in protein abundance and levels of 3-nitrotyrosine, a marker of macrophage stimulation. Proteins of interest were identified using peptide mass fingerprinting. Compared to plasma from sham-exposed rats, plasma from environmental heat- or millimeter wave-exposed rats increased the expression of 11 proteins, and levels of 3-nitrotyrosine in seven proteins, in the NR8383 cells. These altered proteins are associated with inflammation, oxidative stress, and energy metabolism. Findings of this study indicate both environmental heat and 35 GHz millimeter wave exposure elicit the release of macrophage-activating mediators into the plasma of rats.

AB - A macrophage assay and proteomic screening were used to investigate the biological activity of soluble factors in the plasma of millimeter wave-exposed rats. NR8383 rat macrophages were incubated for 24 h with 10% plasma from male Sprague-Dawley rats that had been exposed to sham conditions, or exposed to 42 °C environmental heat or 35 GHz millimeter waves at 75 mW/cm2 until core temperature reached 41.0 °C. Two-dimensional polyacrylamide gel electrophoresis, image analysis, and Western blotting were used to analyze approximately 600 protein spots in the cell lysates for changes in protein abundance and levels of 3-nitrotyrosine, a marker of macrophage stimulation. Proteins of interest were identified using peptide mass fingerprinting. Compared to plasma from sham-exposed rats, plasma from environmental heat- or millimeter wave-exposed rats increased the expression of 11 proteins, and levels of 3-nitrotyrosine in seven proteins, in the NR8383 cells. These altered proteins are associated with inflammation, oxidative stress, and energy metabolism. Findings of this study indicate both environmental heat and 35 GHz millimeter wave exposure elicit the release of macrophage-activating mediators into the plasma of rats.

KW - 3-nitrotyrosine

KW - Oxidative stress

KW - Proteomics

KW - Radiofrequency

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=78349241384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78349241384&partnerID=8YFLogxK

U2 - 10.1002/bem.20598

DO - 10.1002/bem.20598

M3 - Article

C2 - 20683908

AN - SCOPUS:78349241384

VL - 31

SP - 656

EP - 663

JO - Bioelectromagnetics

JF - Bioelectromagnetics

SN - 0197-8462

IS - 8

ER -