Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease

Xianyin Lai, Bonnie L. Blazer-Yost, Vincent H. Gattone, Monalisa N. Muchatuta, Frank Witzmann

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.

Original languageEnglish
Pages (from-to)3775-3782
Number of pages8
JournalProteomics
Volume9
Issue number14
DOIs
StatePublished - Jul 2009

Fingerprint

Autosomal Recessive Polycystic Kidney
Cyst Fluid
Liver
Common Hepatic Duct
Ducts
Fluids
Chemical analysis
Choledochal Cyst
Polycystic Kidney Diseases
Proteins
Pathology
Bile Ducts
Proteomics
Disease Progression
Cysts
Animals
Fibrosis
Animal Models
Cell Proliferation
Databases

Keywords

  • Autosomal recessive polycystic kidney disease
  • LC-MS/MS
  • Liver cyst fluid

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease. / Lai, Xianyin; Blazer-Yost, Bonnie L.; Gattone, Vincent H.; Muchatuta, Monalisa N.; Witzmann, Frank.

In: Proteomics, Vol. 9, No. 14, 07.2009, p. 3775-3782.

Research output: Contribution to journalArticle

Lai, Xianyin ; Blazer-Yost, Bonnie L. ; Gattone, Vincent H. ; Muchatuta, Monalisa N. ; Witzmann, Frank. / Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease. In: Proteomics. 2009 ; Vol. 9, No. 14. pp. 3775-3782.
@article{4f13c42b37ab415ca3812b457cc4e4a0,
title = "Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease",
abstract = "Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.",
keywords = "Autosomal recessive polycystic kidney disease, LC-MS/MS, Liver cyst fluid",
author = "Xianyin Lai and Blazer-Yost, {Bonnie L.} and Gattone, {Vincent H.} and Muchatuta, {Monalisa N.} and Frank Witzmann",
year = "2009",
month = "7",
doi = "10.1002/pmic.200800379",
language = "English",
volume = "9",
pages = "3775--3782",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "14",

}

TY - JOUR

T1 - Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease

AU - Lai, Xianyin

AU - Blazer-Yost, Bonnie L.

AU - Gattone, Vincent H.

AU - Muchatuta, Monalisa N.

AU - Witzmann, Frank

PY - 2009/7

Y1 - 2009/7

N2 - Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.

AB - Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.

KW - Autosomal recessive polycystic kidney disease

KW - LC-MS/MS

KW - Liver cyst fluid

UR - http://www.scopus.com/inward/record.url?scp=68049090011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68049090011&partnerID=8YFLogxK

U2 - 10.1002/pmic.200800379

DO - 10.1002/pmic.200800379

M3 - Article

C2 - 19639592

AN - SCOPUS:68049090011

VL - 9

SP - 3775

EP - 3782

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 14

ER -