Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease

Xianyin Lai, Bonnie L. Blazer-Yost, Vincent H. Gattone, Monalisa N. Muchatuta, Frank A. Witzmann

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Cysts arising from hepatic bile ducts are a common extra-renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c-cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC-MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.

Original languageEnglish (US)
Pages (from-to)3775-3782
Number of pages8
JournalProteomics
Volume9
Issue number14
DOIs
StatePublished - Jul 1 2009

Keywords

  • Autosomal recessive polycystic kidney disease
  • LC-MS/MS
  • Liver cyst fluid

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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