Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol

Richard Bell, M. W. Kimpel, Zachary Rodd, W. N. Strother, F. Bai, C. L. Peper, R. D. Mayfield, L. Lumeng, David Crabb, W. J. McBride, Frank Witzmann

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Chronic ethanol (EtOH) drinking produces neuronal alterations within the limbic system. To investigate changes in protein expression levels associated with EtOH drinking, inbred alcohol-preferring (iP) rats were given one of three EtOH access conditions in their home-cages: continuous ethanol (CE: 24 h/day, 7 days/week access to EtOH), multiple scheduled access (MSA: four 1-h sessions during the dark cycle/day, 5 days/week) to EtOH, or remained EtOH-naïve. Both MSA and CE groups consumed between 6 and 6.5 g of EtOH/kg/day after the 3rd week of access. On the first day of EtOH access for the seventh week, access was terminated at the end of the fourth MSA session for MSA rats and the corresponding time point (2300 h) for CE rats. Ten h later, the rats were decapitated, brains extracted, the nucleus accumbens (NAcc) and amygdala (AMYG) microdissected, and protein isolated for 2-dimensional gel electrophoretic analyses. In the NAcc, MSA altered expression levels for 12 of the 14 identified proteins, compared with controls, with six of these proteins altered by CE access, as well. In the AMYG, CE access changed expression levels for 22 of the 27 identified proteins, compared with controls, with 8 of these proteins altered by MSA, as well. The proteins could be grouped into functional categories of chaperones, cytoskeleton, intracellular communication, membrane transport, metabolism, energy production, or neurotransmission. Overall, it appears that EtOH drinking and the conditions under which EtOH is consumed, differentially affect protein expression levels between the NAcc and AMYG. This may reflect differences in neuroanatomical and/or functional characteristics associated with EtOH self-administration and possibly withdrawal, between these two brain structures.

Original languageEnglish
Pages (from-to)3-17
Number of pages15
JournalAlcohol
Volume40
Issue number1
DOIs
StatePublished - Aug 2006

Fingerprint

Nucleus Accumbens
Amygdala
Rats
brain
Ethanol
alcohol
Alcohols
self-administration
energy production
withdrawal
Proteins
communication
Drinking
Brain
Group
Merozoite Surface Protein 1
Limbic System
Intracellular Membranes
Self Administration
Cytoskeleton

Keywords

  • 2DGE
  • Alcohol drinking
  • Central nervous system
  • Mass spectrometry
  • Proteomics
  • Reinforcement
  • Reward
  • Selective breeding

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol. / Bell, Richard; Kimpel, M. W.; Rodd, Zachary; Strother, W. N.; Bai, F.; Peper, C. L.; Mayfield, R. D.; Lumeng, L.; Crabb, David; McBride, W. J.; Witzmann, Frank.

In: Alcohol, Vol. 40, No. 1, 08.2006, p. 3-17.

Research output: Contribution to journalArticle

Bell, Richard ; Kimpel, M. W. ; Rodd, Zachary ; Strother, W. N. ; Bai, F. ; Peper, C. L. ; Mayfield, R. D. ; Lumeng, L. ; Crabb, David ; McBride, W. J. ; Witzmann, Frank. / Protein expression changes in the nucleus accumbens and amygdala of inbred alcohol-preferring rats given either continuous or scheduled access to ethanol. In: Alcohol. 2006 ; Vol. 40, No. 1. pp. 3-17.
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abstract = "Chronic ethanol (EtOH) drinking produces neuronal alterations within the limbic system. To investigate changes in protein expression levels associated with EtOH drinking, inbred alcohol-preferring (iP) rats were given one of three EtOH access conditions in their home-cages: continuous ethanol (CE: 24 h/day, 7 days/week access to EtOH), multiple scheduled access (MSA: four 1-h sessions during the dark cycle/day, 5 days/week) to EtOH, or remained EtOH-na{\"i}ve. Both MSA and CE groups consumed between 6 and 6.5 g of EtOH/kg/day after the 3rd week of access. On the first day of EtOH access for the seventh week, access was terminated at the end of the fourth MSA session for MSA rats and the corresponding time point (2300 h) for CE rats. Ten h later, the rats were decapitated, brains extracted, the nucleus accumbens (NAcc) and amygdala (AMYG) microdissected, and protein isolated for 2-dimensional gel electrophoretic analyses. In the NAcc, MSA altered expression levels for 12 of the 14 identified proteins, compared with controls, with six of these proteins altered by CE access, as well. In the AMYG, CE access changed expression levels for 22 of the 27 identified proteins, compared with controls, with 8 of these proteins altered by MSA, as well. The proteins could be grouped into functional categories of chaperones, cytoskeleton, intracellular communication, membrane transport, metabolism, energy production, or neurotransmission. Overall, it appears that EtOH drinking and the conditions under which EtOH is consumed, differentially affect protein expression levels between the NAcc and AMYG. This may reflect differences in neuroanatomical and/or functional characteristics associated with EtOH self-administration and possibly withdrawal, between these two brain structures.",
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AU - Bai, F.

AU - Peper, C. L.

AU - Mayfield, R. D.

AU - Lumeng, L.

AU - Crabb, David

AU - McBride, W. J.

AU - Witzmann, Frank

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