Protein intrinsic disorder toolbox for comparative analysis of viral proteins

Gerard Kian Meng Goh, A. Keith Dunker, Vladimir N. Uversky

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

To examine the usefulness of protein disorder predictions as a tool for the comparative analysis of viral proteins, a relational database has been constructed. The database includes proteins from influenza A and HIV-related viruses. Annotations include viral protein sequence, disorder prediction, structure, and function. Location of each protein within a virion, if known, is also denoted. Our analysis reveals a clear relationship between proximity to the RNA core and the percentage of predicted disordered residues for a set of influenza A virus proteins. Neuraminidases (NA) and hemagglutinin (HA) of major influenza A pandemics tend to pair in such a way that both proteins tend to be either ordered-ordered or disordered-disordered by prediction. This may be the result of these proteins evolving from being lipid-associated. High abundance of intrinsic disorder in envelope and matrix proteins from HIV-related viruses likely represents a mechanism where HIV virions can escape immune response despite the availability of antibodies for the HIV-related proteins. This exercise provides an example showing how the combined use of intrinsic disorder predictions and relational databases provides an improved understanding of the functional and structural behaviour of viral proteins.

Original languageEnglish (US)
Article numberS4
JournalBMC genomics
Volume9
Issue numberSUPPL. 2
DOIs
StatePublished - Sep 16 2008

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ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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