Protein kinase A-mediated phosphorylation regulates STAT3 activation and oncogenic EZH2 activity

Ali R. Özeş, Nick Pulliam, Mustafa G. Ertosun, Özlem Yılmaz, Jessica Tang, Ece Çopuroğlu, Daniela Matei, Osman N. Özeş, Kenneth Nephew

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polycomb repressive complex 2 (PRC2) member enhancer of zeste homolog 2 (EZH2) trimethylates histone H3 lysine 27 (H3K27me3), alters chromatin structure and contributes to epigenetic regulation of gene expression in normal and disease processes. Phosphorylation of EZH2 augmented EZH2 oncogenic activity in cancer but observations have been limited to threonine 350 (T350) and serine 21 (S21) residues by cyclin-dependent kinase 1 and protein kinase B, respectively. In addition, phosphorylation of the evolutionarily conserved T372 motif of EZH2 by p38 resulted in EZH2 interaction with Ying Yang 1 and promoted muscle stem cell differentiation. In the present study, we used epithelial ovarian cancer (OC) cells as a model to demonstrate that phosphorylation of EZH2 at T372 by protein kinase A (PKA) induced a dominant-negative EZH2 phenotype, inhibited OC cell proliferation and migration in vitro and decreased ovarian xenograft tumor growth in vivo. Phosphorylation of T372 by PKA enhanced the interaction between EZH2 and signal transducer and activator of transcription 3 (STAT3), and STAT3 binding to pT372-EZH2 reduced cellular levels of pSTAT3 and downregulated interleukin 6 receptor expression in OC. Furthermore, PKA-mediated pT372-EZH2 decreased ATP levels and altered mitochondrial gene expression, resulting in mitochondrial dysfunction and reduced OC cell growth. These findings demonstrate that PKA-mediated T372 phosphorylation reduces oncogenic EZH2 activity and reveal a novel role for pT372 in regulating EZH2 in OC and possibly other cancers.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalOncogene
DOIs
StateAccepted/In press - Mar 26 2018

Fingerprint

STAT3 Transcription Factor
Cyclic AMP-Dependent Protein Kinases
Transcriptional Activation
Phosphorylation
Ovarian Neoplasms
Enhancer of Zeste Homolog 2 Protein
Polycomb Repressive Complex 2
CDC2 Protein Kinase
Interleukin-6 Receptors
Neoplasms
Proto-Oncogene Proteins c-akt
Mitochondrial Genes
Gene Expression Regulation
Threonine
Growth
Heterografts
Epigenomics
Histones
Muscle Cells
Serine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Özeş, A. R., Pulliam, N., Ertosun, M. G., Yılmaz, Ö., Tang, J., Çopuroğlu, E., ... Nephew, K. (Accepted/In press). Protein kinase A-mediated phosphorylation regulates STAT3 activation and oncogenic EZH2 activity. Oncogene, 1-12. https://doi.org/10.1038/s41388-018-0218-z

Protein kinase A-mediated phosphorylation regulates STAT3 activation and oncogenic EZH2 activity. / Özeş, Ali R.; Pulliam, Nick; Ertosun, Mustafa G.; Yılmaz, Özlem; Tang, Jessica; Çopuroğlu, Ece; Matei, Daniela; Özeş, Osman N.; Nephew, Kenneth.

In: Oncogene, 26.03.2018, p. 1-12.

Research output: Contribution to journalArticle

Özeş AR, Pulliam N, Ertosun MG, Yılmaz Ö, Tang J, Çopuroğlu E et al. Protein kinase A-mediated phosphorylation regulates STAT3 activation and oncogenic EZH2 activity. Oncogene. 2018 Mar 26;1-12. https://doi.org/10.1038/s41388-018-0218-z
Özeş, Ali R. ; Pulliam, Nick ; Ertosun, Mustafa G. ; Yılmaz, Özlem ; Tang, Jessica ; Çopuroğlu, Ece ; Matei, Daniela ; Özeş, Osman N. ; Nephew, Kenneth. / Protein kinase A-mediated phosphorylation regulates STAT3 activation and oncogenic EZH2 activity. In: Oncogene. 2018 ; pp. 1-12.
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