Protein kinase A modulates an endogenous calcium channel, but not the calcium-activated chloride channel, in Xenopus oocytes

Yan Chen, Jonathan D. Pollock, Yuhuan Wang, Anna De Paoli-Roach, Lei Yu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In Xenopus oocytes, Ca2+ influx through an endogenous voltage-gated Ca2+ channel activates a transient outward Cl- current (ICI(Ca)), which is potentiated by cAMP increase. The site of cAMP effect appears to be the Ca2+ channel instead of the Ca2+-activated Cl- channel, because cAMP potentiates the Ba2+ current through the Ca2+ channel in a similar way to the ICl(Ca), and cAMP does not potentiate the Ca2+-dependent CI- current in cells treated with Ca2+ ionophore. Using the catalytic subunit of protein kinase A (PKA) and PKA inhibitors, it was shown that PKA is both necessary and sufficient for the cAMP effect on ICI(Ca). Furthermore, the cAMP/PKA-mediated potentiation of IcI(Ca) was inhibited by both type 1 and type 2A protein phosphatases.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalFEBS Letters
Volume336
Issue number2
DOIs
StatePublished - Dec 27 1993

Fingerprint

Chloride Channels
Calcium Channels
Xenopus
Cyclic AMP-Dependent Protein Kinases
Oocytes
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
Protein Phosphatase 1
Protein Phosphatase 2
Ionophores
Protein Kinase Inhibitors
Electric potential

Keywords

  • Calcium channel
  • Calcium ionophore
  • Phosphatase
  • Protein kinase A
  • Xenopus oocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Protein kinase A modulates an endogenous calcium channel, but not the calcium-activated chloride channel, in Xenopus oocytes. / Chen, Yan; Pollock, Jonathan D.; Wang, Yuhuan; De Paoli-Roach, Anna; Yu, Lei.

In: FEBS Letters, Vol. 336, No. 2, 27.12.1993, p. 191-196.

Research output: Contribution to journalArticle

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AU - Yu, Lei

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