Protein kinase C-α and -ε modulate connexin-43 phosphorylation in human heart

Nancy Bowling, Xiao Di Huang, George Sandusky, Rebecca L. Fouts, Karen Mintze, Michail Esterman, Paul D. Allen, Rosemarie Maddi, Eileen McCall, Chris J. Vlahos

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We have previously demonstrated that protein kinase C (PKC)-α expression is significantly elevated in failing human left ventricle, with immunostaining showing increased PKC-α localization at the intercalated disks of cardiomyocytes. In the present study we sought to determine, in the failing heart, if PKC-α interacted with connexin-43 (Cx-43) both spatially and functionally, and to compare the association of PKC-α/Cx-43 with that of PKC-ε, a PKC isozyme that does not significantly increase in failing hearts. The possibility of a PKC-α or PKC-ε/Cx-43 association in non-failing hearts was also investigated. Co-immunoprecipitation of PKC-α or PKC-ε and Cx-43 in non-failing and failing left ventricle was achieved using antibodies to PKC-α or Cx-43. Confocal microscopy confirmed that PKC-α distribution within the cardiomyocyte included co-localization with connexin-43 in both falling and non-failing myocardium. In a similar manner, confocal imaging of PKC-ε showed cardiomyocyte distribution in both cytosol and membrane, and colocalization of PKC-ε with Cx-43. Recombinant PKC-α or -ε increased PKC activity significantly above endogenous levels in the co-immunoprecipitated Cx-43 complexes (P

Original languageEnglish (US)
Pages (from-to)789-798
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume33
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Connexin 43
Protein Kinase C
Phosphorylation
Cardiac Myocytes
Heart Ventricles
Accidental Falls
Immunoprecipitation
Recombinant Proteins
Confocal Microscopy
Cytosol
Isoenzymes

Keywords

  • Cardiomyopathy
  • Gap junctions
  • Phosphoprotein
  • Protein kinase
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Protein kinase C-α and -ε modulate connexin-43 phosphorylation in human heart. / Bowling, Nancy; Huang, Xiao Di; Sandusky, George; Fouts, Rebecca L.; Mintze, Karen; Esterman, Michail; Allen, Paul D.; Maddi, Rosemarie; McCall, Eileen; Vlahos, Chris J.

In: Journal of Molecular and Cellular Cardiology, Vol. 33, No. 4, 2001, p. 789-798.

Research output: Contribution to journalArticle

Bowling, N, Huang, XD, Sandusky, G, Fouts, RL, Mintze, K, Esterman, M, Allen, PD, Maddi, R, McCall, E & Vlahos, CJ 2001, 'Protein kinase C-α and -ε modulate connexin-43 phosphorylation in human heart', Journal of Molecular and Cellular Cardiology, vol. 33, no. 4, pp. 789-798. https://doi.org/10.1006/jmcc.2000.1349
Bowling, Nancy ; Huang, Xiao Di ; Sandusky, George ; Fouts, Rebecca L. ; Mintze, Karen ; Esterman, Michail ; Allen, Paul D. ; Maddi, Rosemarie ; McCall, Eileen ; Vlahos, Chris J. / Protein kinase C-α and -ε modulate connexin-43 phosphorylation in human heart. In: Journal of Molecular and Cellular Cardiology. 2001 ; Vol. 33, No. 4. pp. 789-798.
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