Protein kinase C δ is a critical regulator of CD1d-mediated antigen presentation

Randy R. Brutkiewicz, Claire A. Willard, Kristin K. Gillett-Heacock, M. Robert Pawlak, Jennifer C. Bailey, Masood A. Khan, Manjula Nagala, Wenjun Du, Jacquelyn Gervay-Hague, Gourapura J. Renukaradhya

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We have recently demonstrated that the p38 and ERK1/2 MAP kinases play reciprocal roles in the control of CDld-mediated antigen presentation. Although the use of specific inhibitors for these pathways clearly had an effect, the effects were not complete, leading to speculations that additional pathways were involved. Here, we show that inhibiting protein kinase C δ (PKCδ) substantially impairs antigen presentation by murine CDldl to NKT cells. This effect was accompanied by marked changes in the intracellular localization of CD1d. Expression of a dominant-negative mutant of PKCδ in CD1d+ cells resulted in nearly undetectable endogenous antigen presentation, substantially impaired CD1d recycling, a decrease in MAPK activation, and a decrease in the ability to present low (but not high) concentrations of α-galactosylceramide at the cell surface. These data strongly suggest that PKCδ is a critical regulator of CD1d-mediated antigen presentation and is involved in multiple steps of the process.

Original languageEnglish (US)
Pages (from-to)2390-2395
Number of pages6
JournalEuropean Journal of Immunology
Volume37
Issue number9
DOIs
StatePublished - Sep 1 2007

Keywords

  • Antigen presentation
  • CD1d
  • Signal transduction

ASJC Scopus subject areas

  • Immunology

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    Brutkiewicz, R. R., Willard, C. A., Gillett-Heacock, K. K., Pawlak, M. R., Bailey, J. C., Khan, M. A., Nagala, M., Du, W., Gervay-Hague, J., & Renukaradhya, G. J. (2007). Protein kinase C δ is a critical regulator of CD1d-mediated antigen presentation. European Journal of Immunology, 37(9), 2390-2395. https://doi.org/10.1002/eji.200737124