Protein kinase C isoenzyme expression in retinal cells

Patrick Moriarty, Alan J. Dickson, Jon T. Erichsen, Mike Boulton

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Protein kinase C (PKC) is involved in both the physiological and pathophysiological processes in the retina and plays an important role in signal transduction. The aim of this study was to determine the PKC isoenzyme profile in three retinal cell types in culture, namely RPE cells, pericytes and retinal microvascular endothelial cells. Confluent cultures were lysed and isoenzyme expression detected by Western blotting. PKC isoenzymes α, β2 and δ were observed for all three cell types while β1 was specific for RPE cells. This study has characterised the PKC isoenzyme profile in three retinal cell types and suggests that defining the cell-specific isoenzyme pattern is an important step in understanding their precise physiological role and regulation in the retina. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalOphthalmic Research
Volume32
Issue number2-3
StatePublished - Mar 2000
Externally publishedYes

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Protein Kinase C
Isoenzymes
Retina
Physiological Phenomena
Pericytes
Signal Transduction
Endothelial Cells
Cell Culture Techniques
Western Blotting

Keywords

  • Protein kinase C (PKC)
  • Retinal microvascular endothelial cells
  • Retinal pericytes
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Moriarty, P., Dickson, A. J., Erichsen, J. T., & Boulton, M. (2000). Protein kinase C isoenzyme expression in retinal cells. Ophthalmic Research, 32(2-3), 57-60.

Protein kinase C isoenzyme expression in retinal cells. / Moriarty, Patrick; Dickson, Alan J.; Erichsen, Jon T.; Boulton, Mike.

In: Ophthalmic Research, Vol. 32, No. 2-3, 03.2000, p. 57-60.

Research output: Contribution to journalArticle

Moriarty, P, Dickson, AJ, Erichsen, JT & Boulton, M 2000, 'Protein kinase C isoenzyme expression in retinal cells', Ophthalmic Research, vol. 32, no. 2-3, pp. 57-60.
Moriarty P, Dickson AJ, Erichsen JT, Boulton M. Protein kinase C isoenzyme expression in retinal cells. Ophthalmic Research. 2000 Mar;32(2-3):57-60.
Moriarty, Patrick ; Dickson, Alan J. ; Erichsen, Jon T. ; Boulton, Mike. / Protein kinase C isoenzyme expression in retinal cells. In: Ophthalmic Research. 2000 ; Vol. 32, No. 2-3. pp. 57-60.
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