Protein patterns and proteins that identify subtypes of glioblastoma multiforme

Makoto Furuta, Robert J. Weil, Alexander Vortmeyer, Steve Huang, Jingqi Lei, Tai Nan Huang, Youn Soo Lee, Deb A. Bhowmick, Irina A. Lubensky, Edward H. Oldfield, Zhengping Zhuang

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Glioblastoma multiforme (GBM) has been subdivided into two types based on clinical and genetic findings: primary tumors, which arise de novo, and secondary tumors, which progress from lower grade gliomas to GBMs. To analyse this dichotomy at the protein level, we employed selective tissue microdissection to obtain pure populations of tumor cells, which we studied using two-dimensional protein gel electrophoresis (2-DGE) and protein sequencing of select target proteins. Protein patterns were analysed in a blinded manner from the clinical and genetic data. 2-DGE clearly identified two distinct populations of tumors. 2-DGE was reproducible and reliable, as multiple samples analysed from the same patient gave identical results. In addition, we isolated and sequenced 11 proteins that were uniquely expressed in either the primary or the secondary GBMs, but not both. We demonstrate that specific proteomic patterns can be reproducibly identified by two-dimensional gel electrophoresis from limited numbers of selectively procured, microdissected tumor cells and that two patterns of GBMs, primary versus secondary, previously distinguished by clinical and genetic differences, can be recognized at the protein level. Proteins that are expressed distinctively may have important implications for the diagnosis, prognosis, and treatment of patients with GBM.

Original languageEnglish (US)
Pages (from-to)6806-6814
Number of pages9
JournalOncogene
Volume23
Issue number40
DOIs
StatePublished - Sep 2 2004
Externally publishedYes

Fingerprint

Glioblastoma
Proteins
Electrophoresis, Gel, Two-Dimensional
Neoplasms
Microdissection
Protein Sequence Analysis
Glioma
Proteomics
Population

Keywords

  • Astrocytoma
  • Malignant glioma
  • Mass spectrometry
  • Microdissection
  • Protein sequencing
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Furuta, M., Weil, R. J., Vortmeyer, A., Huang, S., Lei, J., Huang, T. N., ... Zhuang, Z. (2004). Protein patterns and proteins that identify subtypes of glioblastoma multiforme. Oncogene, 23(40), 6806-6814. https://doi.org/10.1038/sj.onc.1207770

Protein patterns and proteins that identify subtypes of glioblastoma multiforme. / Furuta, Makoto; Weil, Robert J.; Vortmeyer, Alexander; Huang, Steve; Lei, Jingqi; Huang, Tai Nan; Lee, Youn Soo; Bhowmick, Deb A.; Lubensky, Irina A.; Oldfield, Edward H.; Zhuang, Zhengping.

In: Oncogene, Vol. 23, No. 40, 02.09.2004, p. 6806-6814.

Research output: Contribution to journalArticle

Furuta, M, Weil, RJ, Vortmeyer, A, Huang, S, Lei, J, Huang, TN, Lee, YS, Bhowmick, DA, Lubensky, IA, Oldfield, EH & Zhuang, Z 2004, 'Protein patterns and proteins that identify subtypes of glioblastoma multiforme', Oncogene, vol. 23, no. 40, pp. 6806-6814. https://doi.org/10.1038/sj.onc.1207770
Furuta, Makoto ; Weil, Robert J. ; Vortmeyer, Alexander ; Huang, Steve ; Lei, Jingqi ; Huang, Tai Nan ; Lee, Youn Soo ; Bhowmick, Deb A. ; Lubensky, Irina A. ; Oldfield, Edward H. ; Zhuang, Zhengping. / Protein patterns and proteins that identify subtypes of glioblastoma multiforme. In: Oncogene. 2004 ; Vol. 23, No. 40. pp. 6806-6814.
@article{fc82e3e044584ecf92974cb4fa2cf4ed,
title = "Protein patterns and proteins that identify subtypes of glioblastoma multiforme",
abstract = "Glioblastoma multiforme (GBM) has been subdivided into two types based on clinical and genetic findings: primary tumors, which arise de novo, and secondary tumors, which progress from lower grade gliomas to GBMs. To analyse this dichotomy at the protein level, we employed selective tissue microdissection to obtain pure populations of tumor cells, which we studied using two-dimensional protein gel electrophoresis (2-DGE) and protein sequencing of select target proteins. Protein patterns were analysed in a blinded manner from the clinical and genetic data. 2-DGE clearly identified two distinct populations of tumors. 2-DGE was reproducible and reliable, as multiple samples analysed from the same patient gave identical results. In addition, we isolated and sequenced 11 proteins that were uniquely expressed in either the primary or the secondary GBMs, but not both. We demonstrate that specific proteomic patterns can be reproducibly identified by two-dimensional gel electrophoresis from limited numbers of selectively procured, microdissected tumor cells and that two patterns of GBMs, primary versus secondary, previously distinguished by clinical and genetic differences, can be recognized at the protein level. Proteins that are expressed distinctively may have important implications for the diagnosis, prognosis, and treatment of patients with GBM.",
keywords = "Astrocytoma, Malignant glioma, Mass spectrometry, Microdissection, Protein sequencing, Two-dimensional gel electrophoresis",
author = "Makoto Furuta and Weil, {Robert J.} and Alexander Vortmeyer and Steve Huang and Jingqi Lei and Huang, {Tai Nan} and Lee, {Youn Soo} and Bhowmick, {Deb A.} and Lubensky, {Irina A.} and Oldfield, {Edward H.} and Zhengping Zhuang",
year = "2004",
month = "9",
day = "2",
doi = "10.1038/sj.onc.1207770",
language = "English (US)",
volume = "23",
pages = "6806--6814",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "40",

}

TY - JOUR

T1 - Protein patterns and proteins that identify subtypes of glioblastoma multiforme

AU - Furuta, Makoto

AU - Weil, Robert J.

AU - Vortmeyer, Alexander

AU - Huang, Steve

AU - Lei, Jingqi

AU - Huang, Tai Nan

AU - Lee, Youn Soo

AU - Bhowmick, Deb A.

AU - Lubensky, Irina A.

AU - Oldfield, Edward H.

AU - Zhuang, Zhengping

PY - 2004/9/2

Y1 - 2004/9/2

N2 - Glioblastoma multiforme (GBM) has been subdivided into two types based on clinical and genetic findings: primary tumors, which arise de novo, and secondary tumors, which progress from lower grade gliomas to GBMs. To analyse this dichotomy at the protein level, we employed selective tissue microdissection to obtain pure populations of tumor cells, which we studied using two-dimensional protein gel electrophoresis (2-DGE) and protein sequencing of select target proteins. Protein patterns were analysed in a blinded manner from the clinical and genetic data. 2-DGE clearly identified two distinct populations of tumors. 2-DGE was reproducible and reliable, as multiple samples analysed from the same patient gave identical results. In addition, we isolated and sequenced 11 proteins that were uniquely expressed in either the primary or the secondary GBMs, but not both. We demonstrate that specific proteomic patterns can be reproducibly identified by two-dimensional gel electrophoresis from limited numbers of selectively procured, microdissected tumor cells and that two patterns of GBMs, primary versus secondary, previously distinguished by clinical and genetic differences, can be recognized at the protein level. Proteins that are expressed distinctively may have important implications for the diagnosis, prognosis, and treatment of patients with GBM.

AB - Glioblastoma multiforme (GBM) has been subdivided into two types based on clinical and genetic findings: primary tumors, which arise de novo, and secondary tumors, which progress from lower grade gliomas to GBMs. To analyse this dichotomy at the protein level, we employed selective tissue microdissection to obtain pure populations of tumor cells, which we studied using two-dimensional protein gel electrophoresis (2-DGE) and protein sequencing of select target proteins. Protein patterns were analysed in a blinded manner from the clinical and genetic data. 2-DGE clearly identified two distinct populations of tumors. 2-DGE was reproducible and reliable, as multiple samples analysed from the same patient gave identical results. In addition, we isolated and sequenced 11 proteins that were uniquely expressed in either the primary or the secondary GBMs, but not both. We demonstrate that specific proteomic patterns can be reproducibly identified by two-dimensional gel electrophoresis from limited numbers of selectively procured, microdissected tumor cells and that two patterns of GBMs, primary versus secondary, previously distinguished by clinical and genetic differences, can be recognized at the protein level. Proteins that are expressed distinctively may have important implications for the diagnosis, prognosis, and treatment of patients with GBM.

KW - Astrocytoma

KW - Malignant glioma

KW - Mass spectrometry

KW - Microdissection

KW - Protein sequencing

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=4644309390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644309390&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1207770

DO - 10.1038/sj.onc.1207770

M3 - Article

VL - 23

SP - 6806

EP - 6814

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 40

ER -