Protein-Specific features associated with variability in human antibody responses to plasmodium falciparum Malaria Antigens

Eugene W. Liu, Jeff Skinner, Tuan  Tran, Krishan Kumar, David L. Narum, Aarti Jain, Aissata Ongoiba, Boubacar Traore, Philip L. Felgner, Peter D. Crompton

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The magnitude of antibody responses varies across the individual proteins that constitute any given microorganism, both in the context of natural infection and vaccination with attenuated or inactivated pathogens. The protein-specific factors underlying this variability are poorly understood. In 267 individuals exposed to intense seasonal malaria, we examined the relationship between immunoglobulin G (IgG) responses to 861 Plasmodium falciparum proteins and specific features of these proteins, including their subcellular location, relative abundance, degree of polymorphism, and whether they are predicted to have human orthologs. We found that IgG reactivity was significantly higher to extracellular and plasma membrane proteins and also correlated positively with both protein abundance and degree of protein polymorphism. Conversely, IgG reactivity was significantly lower to proteins predicted to have human orthologs. These findings provide insight into protein-specific factors that are associated with variability in the magnitude of antibody responses to natural P. falciparum infection-data that could inform vaccine strategies to optimize antibody-mediated immunity as well as the selection of antigens for sero-diagnostic purposes.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume98
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Falciparum Malaria
Antibody Formation
Antigens
Proteins
Immunoglobulin G
Plasmodium falciparum
Malaria
Blood Proteins
Immunity
Membrane Proteins
Vaccination
Vaccines
Cell Membrane
Antibodies
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Protein-Specific features associated with variability in human antibody responses to plasmodium falciparum Malaria Antigens. / Liu, Eugene W.; Skinner, Jeff; Tran, Tuan ; Kumar, Krishan; Narum, David L.; Jain, Aarti; Ongoiba, Aissata; Traore, Boubacar; Felgner, Philip L.; Crompton, Peter D.

In: American Journal of Tropical Medicine and Hygiene, Vol. 98, No. 1, 01.01.2018, p. 57-66.

Research output: Contribution to journalArticle

Liu, Eugene W. ; Skinner, Jeff ; Tran, Tuan  ; Kumar, Krishan ; Narum, David L. ; Jain, Aarti ; Ongoiba, Aissata ; Traore, Boubacar ; Felgner, Philip L. ; Crompton, Peter D. / Protein-Specific features associated with variability in human antibody responses to plasmodium falciparum Malaria Antigens. In: American Journal of Tropical Medicine and Hygiene. 2018 ; Vol. 98, No. 1. pp. 57-66.
@article{8572a2d9d61e4b64a257255796cc0435,
title = "Protein-Specific features associated with variability in human antibody responses to plasmodium falciparum Malaria Antigens",
abstract = "The magnitude of antibody responses varies across the individual proteins that constitute any given microorganism, both in the context of natural infection and vaccination with attenuated or inactivated pathogens. The protein-specific factors underlying this variability are poorly understood. In 267 individuals exposed to intense seasonal malaria, we examined the relationship between immunoglobulin G (IgG) responses to 861 Plasmodium falciparum proteins and specific features of these proteins, including their subcellular location, relative abundance, degree of polymorphism, and whether they are predicted to have human orthologs. We found that IgG reactivity was significantly higher to extracellular and plasma membrane proteins and also correlated positively with both protein abundance and degree of protein polymorphism. Conversely, IgG reactivity was significantly lower to proteins predicted to have human orthologs. These findings provide insight into protein-specific factors that are associated with variability in the magnitude of antibody responses to natural P. falciparum infection-data that could inform vaccine strategies to optimize antibody-mediated immunity as well as the selection of antigens for sero-diagnostic purposes.",
author = "Liu, {Eugene W.} and Jeff Skinner and Tuan  Tran and Krishan Kumar and Narum, {David L.} and Aarti Jain and Aissata Ongoiba and Boubacar Traore and Felgner, {Philip L.} and Crompton, {Peter D.}",
year = "2018",
month = "1",
day = "1",
doi = "10.4269/ajtmh.17-0437",
language = "English (US)",
volume = "98",
pages = "57--66",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "1",

}

TY - JOUR

T1 - Protein-Specific features associated with variability in human antibody responses to plasmodium falciparum Malaria Antigens

AU - Liu, Eugene W.

AU - Skinner, Jeff

AU - Tran, Tuan 

AU - Kumar, Krishan

AU - Narum, David L.

AU - Jain, Aarti

AU - Ongoiba, Aissata

AU - Traore, Boubacar

AU - Felgner, Philip L.

AU - Crompton, Peter D.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The magnitude of antibody responses varies across the individual proteins that constitute any given microorganism, both in the context of natural infection and vaccination with attenuated or inactivated pathogens. The protein-specific factors underlying this variability are poorly understood. In 267 individuals exposed to intense seasonal malaria, we examined the relationship between immunoglobulin G (IgG) responses to 861 Plasmodium falciparum proteins and specific features of these proteins, including their subcellular location, relative abundance, degree of polymorphism, and whether they are predicted to have human orthologs. We found that IgG reactivity was significantly higher to extracellular and plasma membrane proteins and also correlated positively with both protein abundance and degree of protein polymorphism. Conversely, IgG reactivity was significantly lower to proteins predicted to have human orthologs. These findings provide insight into protein-specific factors that are associated with variability in the magnitude of antibody responses to natural P. falciparum infection-data that could inform vaccine strategies to optimize antibody-mediated immunity as well as the selection of antigens for sero-diagnostic purposes.

AB - The magnitude of antibody responses varies across the individual proteins that constitute any given microorganism, both in the context of natural infection and vaccination with attenuated or inactivated pathogens. The protein-specific factors underlying this variability are poorly understood. In 267 individuals exposed to intense seasonal malaria, we examined the relationship between immunoglobulin G (IgG) responses to 861 Plasmodium falciparum proteins and specific features of these proteins, including their subcellular location, relative abundance, degree of polymorphism, and whether they are predicted to have human orthologs. We found that IgG reactivity was significantly higher to extracellular and plasma membrane proteins and also correlated positively with both protein abundance and degree of protein polymorphism. Conversely, IgG reactivity was significantly lower to proteins predicted to have human orthologs. These findings provide insight into protein-specific factors that are associated with variability in the magnitude of antibody responses to natural P. falciparum infection-data that could inform vaccine strategies to optimize antibody-mediated immunity as well as the selection of antigens for sero-diagnostic purposes.

UR - http://www.scopus.com/inward/record.url?scp=85040533275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040533275&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.17-0437

DO - 10.4269/ajtmh.17-0437

M3 - Article

C2 - 29141757

AN - SCOPUS:85040533275

VL - 98

SP - 57

EP - 66

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 1

ER -