Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase

Patricia M. Kane, Carl T. Yamashiro, David F. Wolczyk, Norma Neff, Mark Goebl, Tom H. Stevens

Research output: Contribution to journalArticle

353 Citations (Scopus)

Abstract

The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H+-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5′ and 3′ thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.

Original languageEnglish
Pages (from-to)651-657
Number of pages7
JournalScience
Volume250
Issue number4981
StatePublished - Nov 2 1990

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Protein Splicing
Adenosine Triphosphatases
Yeasts
Genes
Proteins
Proton-Translocating ATPases
Saccharomyces cerevisiae
Protons
Catalytic Domain

ASJC Scopus subject areas

  • General

Cite this

Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., & Stevens, T. H. (1990). Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase. Science, 250(4981), 651-657.

Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase. / Kane, Patricia M.; Yamashiro, Carl T.; Wolczyk, David F.; Neff, Norma; Goebl, Mark; Stevens, Tom H.

In: Science, Vol. 250, No. 4981, 02.11.1990, p. 651-657.

Research output: Contribution to journalArticle

Kane, PM, Yamashiro, CT, Wolczyk, DF, Neff, N, Goebl, M & Stevens, TH 1990, 'Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase', Science, vol. 250, no. 4981, pp. 651-657.
Kane, Patricia M. ; Yamashiro, Carl T. ; Wolczyk, David F. ; Neff, Norma ; Goebl, Mark ; Stevens, Tom H. / Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase. In: Science. 1990 ; Vol. 250, No. 4981. pp. 651-657.
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