Protein synthesis in the newt regenerating limb: Comparative two-dimensional PAGE, computer analysis and protein sequencing

P. A. Tsonis, Anthony Mescher, K. Del Rio-Tsonis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Protein synthesis has been studied by two-dimensional PAGE during the early limb regeneration in the adult newt. Quantitative and statistical analyses have provided unique information on overall patterns of protein synthesis as well as on specific protein synthesis during formation of the blastema. Furthermore, from the patterns in the two-dimensional gels and their quantification, a particular protein has been selected and sequenced. Partial sequencing revealed sequence similarities to Xenopus type I keratin B2. Expression of this keratin is 10-fold greater in the blastema than in the intact limb. The implications of keratin expression by the blastema cells are discussed.

Original languageEnglish (US)
Pages (from-to)665-668
Number of pages4
JournalBiochemical Journal
Volume281
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Salamandridae
Protein Sequence Analysis
Extremities
Keratins
Proteins
Type I Keratin
Keratin-10
Xenopus
Regeneration
Gels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Protein synthesis in the newt regenerating limb : Comparative two-dimensional PAGE, computer analysis and protein sequencing. / Tsonis, P. A.; Mescher, Anthony; Del Rio-Tsonis, K.

In: Biochemical Journal, Vol. 281, No. 3, 1992, p. 665-668.

Research output: Contribution to journalArticle

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