Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors

Michihiro Kobayashi, Sarah c. Nabinger, Yunpeng Bai, Momoko Yoshimoto, Rui Gao, Sisi Chen, Chonghua Yao, Yuanshu Dong, Lujuan Zhang, Sonia Rodriguez, Yumi Yashiro-Ohtani, Warren S. Pear, Nadia Carlesso, Mervin Yoder, Reuben Kapur, Mark Kaplan, Hugo Daniel Lacorazza, Zhong-Yin Zhang, Yan Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The molecular pathways regulating lymphoid priming, fate, and development of multipotent bone marrow hematopoietic stem and progenitor cells (HSPCs) that continuously feed thymic progenitors remain largely unknown. While Notch signal is indispensable for T cell specification and differentiation, the downstream effectors are not well understood. PRL2, a protein tyrosine phosphatase that regulates hematopoietic stem cell proliferation and self-renewal, is highly expressed in murine thymocyte progenitors. Here we demonstrate that protein tyrosine phosphatase PRL2 and receptor tyrosine kinase c-Kit are critical downstream targets and effectors of the canonical Notch/RBPJ pathway in early T cell progenitors. While PRL2 deficiency resulted in moderate defects of thymopoiesis in the steady state, de novo generation of T cells from Prl2 null hematopoietic stem cells was significantly reduced following transplantation. Prl2 null HSPCs also showed impaired T cell differentiation in vitro. We found that Notch/RBPJ signaling upregulated PRL2 as well as c-Kit expression in T cell progenitors. Further, PRL2 sustains Notch-mediated c-Kit expression and enhances stem cell factor/c-Kit signaling in T cell progenitors, promoting effective DN1-DN2 transition. Thus, we have identified a critical role for PRL2 phosphatase in mediating Notch and c-Kit signals in early T cell progenitors. Stem Cells 2017;35:1053–1064.

Original languageEnglish (US)
Pages (from-to)1053-1064
Number of pages12
JournalStem Cells
Volume35
Issue number4
DOIs
StatePublished - Apr 1 2017

Fingerprint

Protein Tyrosine Phosphatases
Hematopoietic Stem Cells
T-Lymphocytes
Cell Differentiation
Stem Cell Factor
Receptor Protein-Tyrosine Kinases
Thymocytes
Phosphoric Monoester Hydrolases
Stem Cells
Transplantation
Bone Marrow
Cell Proliferation

Keywords

  • c-Kit
  • Early T lineage progenitors
  • Notch
  • PRL2
  • T cell progenitors
  • Thymopoiesis

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Kobayashi, M., Nabinger, S. C., Bai, Y., Yoshimoto, M., Gao, R., Chen, S., ... Liu, Y. (2017). Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors. Stem Cells, 35(4), 1053-1064. https://doi.org/10.1002/stem.2559

Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors. / Kobayashi, Michihiro; Nabinger, Sarah c.; Bai, Yunpeng; Yoshimoto, Momoko; Gao, Rui; Chen, Sisi; Yao, Chonghua; Dong, Yuanshu; Zhang, Lujuan; Rodriguez, Sonia; Yashiro-Ohtani, Yumi; Pear, Warren S.; Carlesso, Nadia; Yoder, Mervin; Kapur, Reuben; Kaplan, Mark; Daniel Lacorazza, Hugo; Zhang, Zhong-Yin; Liu, Yan.

In: Stem Cells, Vol. 35, No. 4, 01.04.2017, p. 1053-1064.

Research output: Contribution to journalArticle

Kobayashi, M, Nabinger, SC, Bai, Y, Yoshimoto, M, Gao, R, Chen, S, Yao, C, Dong, Y, Zhang, L, Rodriguez, S, Yashiro-Ohtani, Y, Pear, WS, Carlesso, N, Yoder, M, Kapur, R, Kaplan, M, Daniel Lacorazza, H, Zhang, Z-Y & Liu, Y 2017, 'Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors', Stem Cells, vol. 35, no. 4, pp. 1053-1064. https://doi.org/10.1002/stem.2559
Kobayashi M, Nabinger SC, Bai Y, Yoshimoto M, Gao R, Chen S et al. Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors. Stem Cells. 2017 Apr 1;35(4):1053-1064. https://doi.org/10.1002/stem.2559
Kobayashi, Michihiro ; Nabinger, Sarah c. ; Bai, Yunpeng ; Yoshimoto, Momoko ; Gao, Rui ; Chen, Sisi ; Yao, Chonghua ; Dong, Yuanshu ; Zhang, Lujuan ; Rodriguez, Sonia ; Yashiro-Ohtani, Yumi ; Pear, Warren S. ; Carlesso, Nadia ; Yoder, Mervin ; Kapur, Reuben ; Kaplan, Mark ; Daniel Lacorazza, Hugo ; Zhang, Zhong-Yin ; Liu, Yan. / Protein Tyrosine Phosphatase PRL2 Mediates Notch and Kit Signals in Early T Cell Progenitors. In: Stem Cells. 2017 ; Vol. 35, No. 4. pp. 1053-1064.
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