Proteins of transcription factor ISGF-3: One gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon α

Chris Schindler, Xin Yuan Fu, Teresa Improta, Ruedi Aebersold, James E. Darnell

Research output: Contribution to journalArticle

497 Citations (Scopus)

Abstract

ISGF-3 is an interferon-dependent positive-acting transcription factor that is cytoplasmically activated, possibly through direct interaction with the interferon receptor. The factor has been purified, its component proteins have been separated, and its peptide sequences have been obtained. From the sequences, degenerate oligonucleotide probes were constructed to screen for cDNA clones. Sequencing of the selected clones shows that the 91- and 84-kDa components represent two forms of a previously unknown (to our knowledge) protein. Several antibodies raised against these proteins prove that they indeed do encode protein components of ISGF-3. This work provides reagents to explore the modification of this cytoplasmically activated transcription factor.

Original languageEnglish (US)
Pages (from-to)7836-7839
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number16
StatePublished - 1992
Externally publishedYes

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Transcription Factor 3
Interferons
Genes
Proteins
Transcription Factors
Clone Cells
Interferon Receptors
Oligonucleotide Probes
Complementary DNA
Peptides
Antibodies

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Proteins of transcription factor ISGF-3 : One gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon α. / Schindler, Chris; Fu, Xin Yuan; Improta, Teresa; Aebersold, Ruedi; Darnell, James E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 16, 1992, p. 7836-7839.

Research output: Contribution to journalArticle

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