Proteome-wide lysine acetylation in cortical astrocytes and alterations that occur during infection with brain parasite Toxoplasma gondii

Anne Bouchut, Aarti R. Chawla, Victoria Jeffers, Andy Hudmon, William Sullivan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role of this widespread PTM has yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey of lysine acetylation in cortical astrocytes, a subtype of glia that is a component of the blood-brain barrier and a key regulator of neuronal function and plasticity. We identified 529 lysine acetylation sites across 304 proteins found in multiple cellular compartments that largely function in RNA processing/transcription, metabolism, chromatin biology, and translation. Two hundred and seventy-seven of the acetylated lysines we identified on 186 proteins have not been reported previously in any other cell type. We also mapped an acetylome of astrocytes infected with the brain parasite, Toxoplasma gondii. It has been shown that infection with T. gondii modulates host cell gene expression, including several lysine acetyltransferase (KAT) and deacetylase (KDAC) genes, suggesting that the host acetylome may also be altered during infection. In the T. gondii-infected astrocytes, we identified 34 proteins exhibiting a level of acetylation >2-fold and 24 with a level of acetylation <2-fold relative to uninfected astrocytes. Our study documents the first acetylome map for cortical astrocytes, uncovers novel lysine acetylation sites, and demonstrates that T. gondii infection produces an altered acetylome.

Original languageEnglish
Article numbere0117966
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 18 2015

Fingerprint

Acetylation
astrocytes
Toxoplasma
acetylation
Proteome
Toxoplasma gondii
proteome
Astrocytes
Lysine
Brain
lysine
Parasites
brain
parasites
Infection
infection
Toxoplasmosis
post-translational modification
Post Translational Protein Processing
Proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Proteome-wide lysine acetylation in cortical astrocytes and alterations that occur during infection with brain parasite Toxoplasma gondii. / Bouchut, Anne; Chawla, Aarti R.; Jeffers, Victoria; Hudmon, Andy; Sullivan, William.

In: PLoS One, Vol. 10, No. 3, e0117966, 18.03.2015.

Research output: Contribution to journalArticle

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