Proteomic analysis of differential protein expression induced by ultraviolet light radiation in HeLa cells

Emily D. Decker, Yinong Zhang, Ross R. Cocklin, Frank Witzmann, Mu Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cells treated with ultraviolet (UV) radiation undergo cell cycle arrest at the S-phase and G1/S boundary, allowing DNA repair to occur. Several proteins such as replication protein A and DNA-dependent protein kinase have been suggested to be involved in UV-induced inhibition of DNA replication. However, the role of these proteins in inhibiting DNA replication remains unknown. Other proteins may play important roles in modulating functions of these proteins in response to UV-irradiation. To understand the broad range of proteins involved in this inhibition, we carried out a systematic study to identify specific proteins involved in UV-induced replication arrest using two-dimensional gel electrophoresis and mass spectrometry. Unique changes in protein expression level for 31 proteins were observed over a 24-hour time course, including calgizzarin, cyclophilin A, and macrophage migration inhibitory factor. The expression level changes of these proteins are dynamically correlated to DNA replication activity, suggesting involvement of these proteins in modulating DNA replication and repair activities. This proteomic approach provides opportunities to gain insights into the mechanism by which DNA replication is inhibited.

Original languageEnglish
Pages (from-to)2019-2027
Number of pages9
JournalProteomics
Volume3
Issue number10
DOIs
StatePublished - Oct 2003

Fingerprint

Ultraviolet Rays
HeLa Cells
Proteomics
Radiation
DNA Replication
Proteins
DNA
DNA Repair
Repair
Replication Protein A
Cyclophilin A
DNA-Activated Protein Kinase
Macrophage Migration-Inhibitory Factors
Electrophoresis, Gel, Two-Dimensional
Cell Cycle Checkpoints
Electrophoresis
S Phase
Ultraviolet radiation
Mass spectrometry
Mass Spectrometry

Keywords

  • DNA replication
  • HeLa cells
  • Two-dimensional gel electrophoresis
  • Ultraviolet irradiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Proteomic analysis of differential protein expression induced by ultraviolet light radiation in HeLa cells. / Decker, Emily D.; Zhang, Yinong; Cocklin, Ross R.; Witzmann, Frank; Wang, Mu.

In: Proteomics, Vol. 3, No. 10, 10.2003, p. 2019-2027.

Research output: Contribution to journalArticle

Decker, Emily D. ; Zhang, Yinong ; Cocklin, Ross R. ; Witzmann, Frank ; Wang, Mu. / Proteomic analysis of differential protein expression induced by ultraviolet light radiation in HeLa cells. In: Proteomics. 2003 ; Vol. 3, No. 10. pp. 2019-2027.
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