Proteomic analysis of hemangioblastoma cyst fluid

Sven Gläsker, Alexander Vortmeyer, Russell R. Lonser, Irina A. Lubensky, Hiroaki Okamoto, John B. Xia, Jie Li, Erin Milne, Jeffrey A. Kowalak, Edward H. Oldfield, Zhengping Zhuang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: Hemangioblastomas are benign CNS tumors that occur sporadically or in patients with von Hippel-Lindau (VHL) disease. These tumors are characteristically associated with formation of intra- or peritumoral cysts. Hemangioblastoma cyst formation is a major cause of morbidity and mortality with these tumors. While peritumoral cysts have been suggested to result from vascular leakage, the mechanism of intratumoral cyst formation is not understood. Methods: To elucidate the origin of intratumoral hemangioblastoma cyst fluid, we characterized its biochemical composition by two-dimensional (2D) proteomic profiling followed by sequencing of several proteins. The proteomic pattern of intratumoral cyst fluid was furthermore compared to the proteomic pattern of serum, hemangioblastoma tumor tissue, and hemangioblastoma peritumoral cyst fluid. Results: We show that proteomic patterns of intra- and peritumoral cyst fluid are identical Both are highly similar to serum and not to tumor. Conclusions: Intratumoral hemangioblastoma cyst fluid originates from serum. Cyst formation associated with hemangioblastoma, whether peri- or intratumoral, is a consequence of vascular leakage. Anti-VEGF therapy may effectively control hemangioblastoma cyst formation.

Original languageEnglish (US)
Pages (from-to)549-553
Number of pages5
JournalCancer Biology and Therapy
Volume5
Issue number5
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Fingerprint

Hemangioblastoma
Cyst Fluid
Proteomics
Cysts
Neoplasms
Blood Vessels
Serum
von Hippel-Lindau Disease
Protein Sequence Analysis
Vascular Endothelial Growth Factor A
Morbidity
Mortality

Keywords

  • Cyst
  • Hemangioblastoma
  • Proteomics
  • Von Hippel-Lindau disease

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Gläsker, S., Vortmeyer, A., Lonser, R. R., Lubensky, I. A., Okamoto, H., Xia, J. B., ... Zhuang, Z. (2006). Proteomic analysis of hemangioblastoma cyst fluid. Cancer Biology and Therapy, 5(5), 549-553. https://doi.org/10.4161/cbt.5.5.2657

Proteomic analysis of hemangioblastoma cyst fluid. / Gläsker, Sven; Vortmeyer, Alexander; Lonser, Russell R.; Lubensky, Irina A.; Okamoto, Hiroaki; Xia, John B.; Li, Jie; Milne, Erin; Kowalak, Jeffrey A.; Oldfield, Edward H.; Zhuang, Zhengping.

In: Cancer Biology and Therapy, Vol. 5, No. 5, 01.01.2006, p. 549-553.

Research output: Contribution to journalArticle

Gläsker, S, Vortmeyer, A, Lonser, RR, Lubensky, IA, Okamoto, H, Xia, JB, Li, J, Milne, E, Kowalak, JA, Oldfield, EH & Zhuang, Z 2006, 'Proteomic analysis of hemangioblastoma cyst fluid', Cancer Biology and Therapy, vol. 5, no. 5, pp. 549-553. https://doi.org/10.4161/cbt.5.5.2657
Gläsker S, Vortmeyer A, Lonser RR, Lubensky IA, Okamoto H, Xia JB et al. Proteomic analysis of hemangioblastoma cyst fluid. Cancer Biology and Therapy. 2006 Jan 1;5(5):549-553. https://doi.org/10.4161/cbt.5.5.2657
Gläsker, Sven ; Vortmeyer, Alexander ; Lonser, Russell R. ; Lubensky, Irina A. ; Okamoto, Hiroaki ; Xia, John B. ; Li, Jie ; Milne, Erin ; Kowalak, Jeffrey A. ; Oldfield, Edward H. ; Zhuang, Zhengping. / Proteomic analysis of hemangioblastoma cyst fluid. In: Cancer Biology and Therapy. 2006 ; Vol. 5, No. 5. pp. 549-553.
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