Proteomic Analysis of Neuroblastoma Microenvironment: Effect of the Host-Tumor Interaction on Disease Progression

Katharyn E. Turner, Hari R. Kumar, Derek J. Hoelz, Xiaoling Zhong, Frederick J. Rescorla, Robert J. Hickey, Linda H. Malkas, John A. Sandoval

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Children with advanced-stage neuroblastoma (NB) traditionally experience poor outcomes. Because early detection of advanced-stage disease may impact survival, finding new targets for early diagnosis is crucial. Evidence suggests the tumor microenvironment may have profound effects on cancer progression. Methods: As little is known concerning the NB-host microenvironment, this study applied proteomic techniques, two-dimensional polyacrylamide gel electrophoresis (2D PAGE) combined with matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry to determine protein differences between cell cultured NB and tumors grown in mice for 2, 4, and 5 wk. Results: We found an increase in proteins in cultured NB compared with implanted mouse tumors during tumor progression. Additionally, analyzing in vivo tumors to cultured NB, we observed less expressed proteins. However, 16 out of 19 proteins were of mouse origin, thus inferring host-derived factors contributing to tumor growth. Conclusion: We show that the dynamic relationship between NB and host microenvironment is important for tumor growth and better understanding of this milieu maybe relevant towards finding unique approaches for identifying advanced-stage disease.

Original languageEnglish (US)
Pages (from-to)116-122
Number of pages7
JournalJournal of Surgical Research
Volume156
Issue number1
DOIs
StatePublished - Sep 1 2009

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Neuroblastoma
Proteomics
Disease Progression
Neoplasms
Electrophoresis, Gel, Two-Dimensional
Cultured Tumor Cells
Proteins
Tumor Microenvironment
Growth
Early Diagnosis
Mass Spectrometry
Lasers

Keywords

  • cancer progression
  • murine tumor model
  • neuroblastoma
  • proteomics
  • tumor microenvironment

ASJC Scopus subject areas

  • Surgery

Cite this

Proteomic Analysis of Neuroblastoma Microenvironment : Effect of the Host-Tumor Interaction on Disease Progression. / Turner, Katharyn E.; Kumar, Hari R.; Hoelz, Derek J.; Zhong, Xiaoling; Rescorla, Frederick J.; Hickey, Robert J.; Malkas, Linda H.; Sandoval, John A.

In: Journal of Surgical Research, Vol. 156, No. 1, 01.09.2009, p. 116-122.

Research output: Contribution to journalArticle

Turner, Katharyn E. ; Kumar, Hari R. ; Hoelz, Derek J. ; Zhong, Xiaoling ; Rescorla, Frederick J. ; Hickey, Robert J. ; Malkas, Linda H. ; Sandoval, John A. / Proteomic Analysis of Neuroblastoma Microenvironment : Effect of the Host-Tumor Interaction on Disease Progression. In: Journal of Surgical Research. 2009 ; Vol. 156, No. 1. pp. 116-122.
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