Proteomic analysis of simulated occupational jet fuel exposure in the lung

Frank Witzmann, Mark D. Bauer, Angela M. Fieno, Raymond A. Grant, Thomas W. Keough, Steven E. Kornguth, Martin P. Lacey, Frank L. Siegel, Yiping Sun, Lynda S. Wright, Robert S. Young, Mark L. Witten

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We analyzed protein expression in the cytosolic fraction prepared from whole lung tissue in male Swiss-Webster mice exposed 1 h/day for seven days to aerosolized JP-8 jet fuel at concentrations of 1000 and 2500 mg/m3, simulating military occupational exposure. Lung cytosol samples were solubilized and separated via large scale, high resolution two-dimensional electrophoresis (2-DE) and gel patterns scanned, digitized and processed for statistical analysis. Significant quantitative and qualitative changes in tissue cytosol proteins resulted from jet fuel exposure. Several of the altered proteins were identified by peptide mass fingerprinting, confirmed by sequence tag analysis, and related to impaired protein synthetic machinery, toxic/metabolic stress and detoxification systems, ultrastructural damage, and functional responses to CO2 handling, acid-base homeostasis and fluid secretion. These results demonstrate a significant but comparatively moderate JP-8 effect on protein expression and corroborate previous morphological and biochemical evidence. Further molecular marker development and mechanistic inferences from these observations await proteomic analysis of whole tissue homogenates and other cell compartment, i.e., mitochondria, microsomes, and nuclei of lung and other targets.

Original languageEnglish
Pages (from-to)3659-3669
Number of pages11
JournalElectrophoresis
Volume20
Issue number18
StatePublished - 1999

Fingerprint

Jet fuel
Proteomics
Lung
Tissue
Proteins
Cytosol
Fluids and Secretions
Detoxification
Physiological Stress
Mitochondria
Peptide Mapping
Poisons
Occupational Exposure
Microsomes
Electrophoresis
Machinery
Sequence Analysis
Statistical methods
Homeostasis
Gels

Keywords

  • Jet fuel
  • Lung
  • Mass spectrometry
  • Mouse
  • Proteomics
  • Toxicity
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Witzmann, F., Bauer, M. D., Fieno, A. M., Grant, R. A., Keough, T. W., Kornguth, S. E., ... Witten, M. L. (1999). Proteomic analysis of simulated occupational jet fuel exposure in the lung. Electrophoresis, 20(18), 3659-3669.

Proteomic analysis of simulated occupational jet fuel exposure in the lung. / Witzmann, Frank; Bauer, Mark D.; Fieno, Angela M.; Grant, Raymond A.; Keough, Thomas W.; Kornguth, Steven E.; Lacey, Martin P.; Siegel, Frank L.; Sun, Yiping; Wright, Lynda S.; Young, Robert S.; Witten, Mark L.

In: Electrophoresis, Vol. 20, No. 18, 1999, p. 3659-3669.

Research output: Contribution to journalArticle

Witzmann, F, Bauer, MD, Fieno, AM, Grant, RA, Keough, TW, Kornguth, SE, Lacey, MP, Siegel, FL, Sun, Y, Wright, LS, Young, RS & Witten, ML 1999, 'Proteomic analysis of simulated occupational jet fuel exposure in the lung', Electrophoresis, vol. 20, no. 18, pp. 3659-3669.
Witzmann F, Bauer MD, Fieno AM, Grant RA, Keough TW, Kornguth SE et al. Proteomic analysis of simulated occupational jet fuel exposure in the lung. Electrophoresis. 1999;20(18):3659-3669.
Witzmann, Frank ; Bauer, Mark D. ; Fieno, Angela M. ; Grant, Raymond A. ; Keough, Thomas W. ; Kornguth, Steven E. ; Lacey, Martin P. ; Siegel, Frank L. ; Sun, Yiping ; Wright, Lynda S. ; Young, Robert S. ; Witten, Mark L. / Proteomic analysis of simulated occupational jet fuel exposure in the lung. In: Electrophoresis. 1999 ; Vol. 20, No. 18. pp. 3659-3669.
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