Proteomic analysis of the renal effects of simulated occupational jet fuel exposure

Frank A. Witzmann, Mark D. Bauer, Angela M. Fieno, Raymond A. Grant, Thomas W. Keough, Martin P. Lacey, Yiping Sun, Mark L. Witten, Robert S. Young

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We analyzed protein expression in the cytosolic fraction prepared from whole kidneys in male Swiss-Webster mice exposed 1 h/day for five days to aerosolized JP-8 jet fuel at a concentration of 1000 mg/m3, simulating military occupational exposure. Kidney cytosol samples were solubilized and separated via large-scale, high-resolution two-dimensional electrophoresis (2-DE) and gel patterns scanned, digitized and processed for statistical analysis. Significant changes in soluble kidney proteins resulted from jet fuel exposure. Several of the altered proteins were identified by peptide mass fingerprinting and related to ultrastructural abnormalities, altered protein processing, metabolic effects, and paradoxical stress protein/detoxification system responses. These results demonstrate a significant but comparatively moderate JP-8 effect on protein expression in the kidney and provide novel molecular evidence of JP-8 nephrotoxicity. Human risk is suggested by these data but conclusive assessment awaits a noninvasive search for biomarkers in JP-8 exposed humans.

Original languageEnglish (US)
Pages (from-to)976-984
Number of pages9
JournalELECTROPHORESIS
Volume21
Issue number5
DOIs
StatePublished - Apr 19 2000

Keywords

  • Jet fuel
  • Kidney
  • Mass spectrometry
  • Mouse
  • Proteomics
  • Toxicity
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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    Witzmann, F. A., Bauer, M. D., Fieno, A. M., Grant, R. A., Keough, T. W., Lacey, M. P., Sun, Y., Witten, M. L., & Young, R. S. (2000). Proteomic analysis of the renal effects of simulated occupational jet fuel exposure. ELECTROPHORESIS, 21(5), 976-984. https://doi.org/10.1002/(SICI)1522-2683(20000301)21:5<976::AID-ELPS976>3.0.CO;2-5